ArticleJournal of bacteriology2011
Membrane topology and DNA-binding ability of the Streptococcal CpsA protein.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- SPD_0410 negatively regulates capsule polysaccharide synthesis and virulence inFrontiers in microbiology · 2024Article
- Encapsulation of the septal cell wall protects Streptococcus pneumoniae from its major peptidoglycan hydrolase and host defenses.PLoS pathogens · 2022Article
- Independent Promoter Recognition by TcpP Precedes Cooperative Promoter Activation by TcpP and ToxR.mBio · 2021Article
- LcpB Is a Pyrophosphatase Responsible for Wall Teichoic Acid Synthesis and Virulence inFrontiers in microbiology · 2021Article
- Polyamine Synthesis Effects Capsule Expression by Reduction of Precursors inFrontiers in microbiology · 2019Article
- In vivo virulence of Mycobacterium tuberculosis depends on a single homologue of the LytR-CpsA-Psr proteins.Scientific reports · 2018Article
- Article
- Expression of BrpA in Streptococcus mutans is regulated by FNR-box mediated repression.Molecular oral microbiology · 2017Article
- Identification of Extracellular Segments by Mass Spectrometry Improves Topology Prediction of Transmembrane Proteins.Scientific reports · 2017Article
- Article
- First human case report of sepsis due to infection with Streptococcus suis serotype 31 in Thailand.BMC infectious diseases · 2015Article
- Sequence elements upstream of the core promoter are necessary for full transcription of the capsule gene operon in Streptococcus pneumoniae strain D39.Infection and immunity · 2015Article
- Streptococcus agalactiae capsule polymer length and attachment is determined by the proteins CpsABCD.The Journal of biological chemistry · 2015Article
- Modification of the CpsA protein reveals a role in alteration of the Streptococcus agalactiae cell envelope.Infection and immunity · 2015Article
- Structural and functional characterization of Pseudomonas aeruginosa global regulator AmpR.Journal of bacteriology · 2014Article
- Deficiency of BrpB causes major defects in cell division, stress responses and biofilm formation by Streptococcus mutans.Microbiology (Reading, England) · 2014Article
- Psr is involved in regulation of glucan production, and double deficiency of BrpA and Psr is lethal in Streptococcus mutans.Microbiology (Reading, England) · 2013Article
- Functional analysis of the CpsA protein of Streptococcus agalactiae.Journal of bacteriology · 2012Article
- BrpA is involved in regulation of cell envelope stress responses in Streptococcus mutans.Applied and environmental microbiology · 2012Article
- Chemical inhibition of bacterial protein tyrosine phosphatase suppresses capsule production.PloS one · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Many streptococcal pathogens require a polysaccharide capsule for survival in the host during systemic infection. The highly conserved CpsA protein is proposed to be a transcriptional regulator of capsule production in streptococci, although the regulatory mechanism is unknown. Hydropathy plots of CpsA predict an integral membrane protein with 3 transmembrane domains and only 27 cytoplasmic residues, whereas other members of the LytR_cpsA_psr protein family are predicted to have a single transmembrane domain. This unique topology, with the short cytoplasmic domain, membrane localization, and large extracellular domain, suggests a novel mechanism of transcriptional regulation. Therefore, to determine the actual membrane topology of CpsA, specific protein domains were fused to beta-galactosidase or alkaline phosphatase. Enzymatic assays confirmed that the predicted membrane topology for CpsA is correct. To investigate how this integral membrane protein may be functioning in regulation of capsule transcription, purified full-length and truncated forms of CpsA were used in electrophoretic mobility shift assays to characterize the ability to bind the capsule operon promoter. Assays revealed that full-length, purified CpsA protein binds specifically to DNA containing the capsule promoter region. Furthermore, the large extracellular domain is not required for DNA binding, but all cytoplasmic regions of CpsA are necessary and sufficient for specific binding to the capsule operon promoter. This is the first demonstration of a member of this protein family interacting with its target DNA. Taken together, CpsA, as well as other members of the LytR_cpsA_psr protein family, appears to utilize a unique mechanism of transcriptional regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.