ArticleJournal of bacteriology2011
Primosomal proteins DnaD and DnaB are recruited to chromosomal regions bound by DnaA in Bacillus subtilis.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 47 citations in OpenAlex.
- Processing of stalled replication forks in Bacillus subtilis.FEMS microbiology reviews · 2024Review
- The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin.Nucleic acids research · 2023Article
- SirA inhibits the essential DnaA:DnaD interaction to block helicase recruitment during Bacillus subtilis sporulation.Nucleic acids research · 2023Article
- Diverse Mechanisms of Helicase Loading during DNA Replication Initiation in Bacteria.Journal of bacteriology · 2023Review
- Biological and Genomic Characteristics of MaMV-DH01, a Novel FreshwaterMicrobiology spectrum · 2023Article
- Topological stress is responsible for the detrimental outcomes of head-on replication-transcription conflicts.Cell reports · 2021Article
- Regulation of DNA Binding and High-Order Oligomerization of the DnaB Helicase Loader.Journal of bacteriology · 2020Article
- Identification of the Unwinding Region in theFrontiers in microbiology · 2020Article
- Blocking the Trigger: Inhibition of the Initiation of Bacterial Chromosome Replication as an Antimicrobial Strategy.Antibiotics (Basel, Switzerland) · 2019Review
- Cryptic protein interactions regulate DNA replication initiation.Molecular microbiology · 2019Article
- Genomic Characterization of Cyanophage vB_AphaS-CL131 Infecting Filamentous Diazotrophic CyanobacteriumApplied and environmental microbiology · 2019Article
- Bacillus subtilis RarA modulates replication restart.Nucleic acids research · 2018Article
- DNA gyrase activity regulates DnaA-dependent replication initiation in Bacillus subtilis.Molecular microbiology · 2018Article
- Genetic networks controlled by the bacterial replication initiator and transcription factor DnaA in Bacillus subtilis.Molecular microbiology · 2017Article
- High-throughput CRISPRi phenotyping identifies new essential genes inMolecular systems biology · 2017Article
- Genetic and biochemical interactions between the bacterial replication initiator DnaA and the nucleoid-associated protein Rok in Bacillus subtilis.Molecular microbiology · 2017Article
- Replication of Staphylococcal Resistance Plasmids.Frontiers in microbiology · 2017Review
- Structural Mechanisms of Hexameric Helicase Loading, Assembly, and Unwinding.F1000Research · 2016Review
- The B. subtilis Accessory Helicase PcrA Facilitates DNA Replication through Transcription Units.PLoS genetics · 2015Article
- In Vitro Whole Genome DNA Binding Analysis of the Bacterial Replication Initiator and Transcription Factor DnaA.PLoS genetics · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The initiation of DNA replication requires the binding of the initiator protein, DnaA, to specific binding sites in the chromosomal origin of replication, oriC. DnaA also binds to many sites around the chromosome, outside oriC, and acts as a transcription factor at several of these. In low-G+C Gram-positive bacteria, the primosomal proteins DnaD and DnaB, in conjunction with loader ATPase DnaI, load the replicative helicase at oriC, and this depends on DnaA. DnaD and DnaB also are required to load the replicative helicase outside oriC during replication restart, independently of DnaA. Using chromatin immunoprecipitation, we found that DnaD and DnaB, but not the replicative helicase, are associated with many of the chromosomal regions bound by DnaA in Bacillus subtilis. This association was dependent on DnaA, and the order of recruitment was the same as that at oriC, but it was independent of a functional oriC and suggests that DnaD and DnaB do not require open complex formation for the stable association with DNA. These secondary binding regions for DnaA could be serving as a reservoir for excess DnaA, DnaD, and DnaB to help properly regulate replication initiation and perhaps are analogous to the proposed function of the datA locus in Escherichia coli. Alternatively, DnaD and DnaB might modulate the activity of DnaA at the secondary binding regions. All three of these proteins are widely conserved and likely have similar functions in a range of organisms.
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