ArticleApplied and environmental microbiology2011
The type II secretion system is essential for erythrocyte lysis and gut colonization by the leech digestive tract symbiont Aeromonas veronii.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 50 citations in OpenAlex.
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- Pan-Genome Analysis of Delftia tsuruhatensis Reveals Important Traits Concerning the Genetic Diversity, Pathogenicity, and Biotechnological Properties of the Species.Microbiology spectrum · 2022Article
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- Marine vampires: Persistent, internal associations between bacteria and blood-feeding marine annelids and crustaceans.Frontiers in microbiology · 2022Article
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- European Medicinal Leeches-New Roles in Modern Medicine.Biomedicines · 2020Review
- CRISPR/Cas9-mediated gene deletion of the ompA gene in symbiotic Cedecea neteri impairs biofilm formation and reduces gut colonization of Aedes aegypti mosquitoes.PLoS neglected tropical diseases · 2019Article
- Comparative genomics of Aeromonas veronii: Identification of a pathotype impacting aquaculture globally.PloS one · 2019Article
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- Endosymbiont genomes yield clues of tubeworm success.The ISME journal · 2018Article
- Potassium is a key signal in host-microbiome dysbiosis in periodontitis.PLoS pathogens · 2017Article
- Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond.Infection and immunity · 2017Review
- Article
- A conserved bacterial protein induces pancreatic beta cell expansion during zebrafish development.eLife · 2016Article
- A Tale of Transmission: Aeromonas veronii Activity within Leech-Exuded Mucus.Applied and environmental microbiology · 2016Article
- Host Matters: Medicinal Leech Digestive-Tract Symbionts and Their Pathogenic Potential.Frontiers in microbiology · 2016Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hemolysin and the type II secretion system (T2SS) have been shown to be important for virulence in many pathogens, but very few studies have shown their importance in beneficial microbes. Here, we investigated the importance of the type II secretion pathway in the beneficial digestive-tract association of Aeromonas veronii and the medicinal leech Hirudo verbana and revealed a critical role for the hemolysis of erythrocytes. A mutant with a miniTn5 insertion in exeM, which is involved in forming the inner membrane platform in the T2SS, was isolated by screening mutants for loss of hemolysis on blood agar plates. A hemolysis assay was used to quantify the mutant's deficiency in lysing sheep erythrocytes and revealed a 99.9% decrease compared to the parent strain. The importance of the T2SS in the colonization of the symbiotic host was assessed. Colonization assays revealed that the T2SS is critical for initial colonization of the leech gut. The defect was tied to the loss of hemolysin production by performing a colonization assay with blood containing lysed erythrocytes. This restored the colonization defect in the mutant. Complementation of the mutant using the promoter region and exeMN revealed that the T2SS is responsible for secreting hemolysin into the extracellular space and that both the T2SS and hemolysin export by the T2SS are critical for initial establishment of A. veronii in the leech gut.
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