ArticleThe Journal of biological chemistry2011
Negative regulation of the endocytic adaptor disabled-2 (Dab2) in mitosis.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- cJun-N-terminal kinase activity and mitosis perturbation drive the 2-methoxyestradiol-mediated enhancement of viral oncolysis by Epizootic Hemorrhagic Disease Virus-Tel Aviv University.Scientific reports · 2025Article
- Inhibition of early EHDV2-Ibaraki infection steps in bovine cells by endosome alkalinization or ikarugamycin, but not by blockage of individual endocytic pathways.Frontiers in cellular and infection microbiology · 2025Article
- Review
- The scaffold protein disabled 2 (DAB2) and its role in tumor development and progression.Molecular biology reports · 2024Review
- Disabled-2 (International journal of molecular sciences · 2022Review
- Integrin αIIbβ3 outside-in signaling activates human platelets through serine 24 phosphorylation of Disabled-2.Cell & bioscience · 2021Article
- ApoER2 (Apolipoprotein E Receptor-2) Deficiency Accelerates Smooth Muscle Cell Senescence via Cytokinesis Impairment and Promotes Fibrotic Neointima After Vascular Injury.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- Clathrin-containing adhesion complexes.The Journal of cell biology · 2019Review
- A unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel.Nature microbiology · 2018Article
- Differential molecular regulation of processing and membrane expression of Type-I BMP receptors: implications for signaling.Cellular and molecular life sciences : CMLS · 2017Article
- Disabled-2 is a negative immune regulator of lipopolysaccharide-stimulated Toll-like receptor 4 internalization and signaling.Scientific reports · 2016Article
- Low Density Lipoprotein Receptor Related Proteins as Regulators of Neural Stem and Progenitor Cell Function.Stem cells international · 2016Review
- Dynamics and restriction of murine leukemia virus cores in mitotic and interphase cells.Retrovirology · 2015Article
- Akt recruits Dab2 to albumin endocytosis in the proximal tubule.American journal of physiology. Renal physiology · 2014Article
- Dab2 inhibits the cholesterol-dependent activation of JNK by TGF-β.Molecular biology of the cell · 2014Article
- An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis.eLife · 2014Article
- Epizootic hemorrhagic disease virus induces and benefits from cell stress, autophagy, and apoptosis.Journal of virology · 2013Article
- Mitotic inhibition of clathrin-mediated endocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- Coated pit-mediated endocytosis of the type I transforming growth factor-β (TGF-β) receptor depends on a di-leucine family signal and is not required for signaling.The Journal of biological chemistry · 2012Article
- Clathrin-mediated endocytosis is inhibited during mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitotic cells undergo extensive changes in shape and size through the altered regulation and function of their membrane trafficking machinery. Disabled 2 (Dab2), a multidomain cargo-specific endocytic adaptor and a mediator of signal transduction, is a potential integrator of trafficking and signaling. Dab2 binds effectors of signaling and trafficking that localize to different intracellular compartments. Thus, differential localization is a putative regulatory mechanism of Dab2 function. Furthermore, Dab2 is phosphorylated in mitosis and is thus regulated in the cell cycle. However, a detailed description of the intracellular localization of Dab2 in the different phases of mitosis and an understanding of the functional consequences of its phosphorylation are lacking. Here, we show that Dab2 is progressively displaced from the membrane in mitosis. This phenomenon is paralleled by a loss of co-localization with clathrin. Both phenomena culminate in metaphase/anaphase and undergo partial recovery in cytokinesis. Treatment with 2-methoxyestradiol, which arrests cells at the spindle assembly checkpoint, induces the same effects observed in metaphase cells. Moreover, 2-methoxyestradiol also induced Dab2 phosphorylation and reduced Dab2/clathrin interactions, endocytic vesicle motility, clathrin exchange dynamics, and the internalization of a receptor endowed with an NPXY endocytic signal. Serine/threonine to alanine mutations, of residues localized to the central region of Dab2, attenuated its phosphorylation, reduced its membrane displacement, and maintained its endocytic abilities in mitosis. We propose that the negative regulation of Dab2 is part of an accommodation of the cell to the altered physicochemical conditions prevalent in mitosis, aimed at allowing endocytic activity throughout the cell cycle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.