ArticleAnalytical biochemistry2011
High-performance liquid chromatography separation and intact mass analysis of detergent-solubilized integral membrane proteins.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- Capillary Zone Electrophoresis-Mass Spectrometry of Intact G Protein-Coupled Receptors Enables Proteoform Profiling.Analytical chemistry · 2025Article
- Affinity Purification of Membrane β-Barrel Proteins via Biotin-Tagged Peptidiscs.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Malabaricone C as Natural Sphingomyelin Synthase Inhibitor against Diet-Induced Obesity and Its Lipid Metabolism in Mice.ACS medicinal chemistry letters · 2019Article
- Rapid LC-MS Method for Accurate Molecular Weight Determination of Membrane and Hydrophobic Proteins.Analytical chemistry · 2018Article
- Top-down mass spectrometry of intact membrane protein complexes reveals oligomeric state and sequence information in a single experiment.Protein science : a publication of the Protein Society · 2015Article
- Reconstitution of membrane protein complexes involved in pneumococcal septal cell wall assembly.PloS one · 2013Article
- The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4.Journal of molecular biology · 2012Article
- Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation.New biotechnology · 2012Article
- Ultra-thin layer MALDI mass spectrometry of membrane proteins in nanodiscs.Analytical and bioanalytical chemistry · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
We have developed a method for intact mass analysis of detergent-solubilized and purified integral membrane proteins using liquid chromatography-mass spectrometry (LC-MS) with methanol as the organic mobile phase. Membrane proteins and detergents are separated chromatographically during the isocratic stage of the gradient profile from a 150-mm C3 reversed-phase column. The mass accuracy is comparable to standard methods employed for soluble proteins; the sensitivity is 10-fold lower, requiring 0.2-5 μg of protein. The method is also compatible with our standard LC-MS method used for intact mass analysis of soluble proteins and may therefore be applied on a multiuser instrument or in a high-throughput environment.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.