ArticleMicrobial cell factories2010
Metabolic response of Geobacter sulfurreducens towards electron donor/acceptor variation.
Article in Microbial cell factories, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 73 citations in OpenAlex.
- Overview of pathways and dynamic interactions of c-type cytochromes in Geobacter sulfurreducens.Biochemical Society transactions · 2026Review
- Environmental considerations and bioremediation applications of the metal-, nitrate-, and organohalide-respiring bacteriumApplied and environmental microbiology · 2026Review
- Propionate oxidation byApplied and environmental microbiology · 2026Article
- The role ofSustainable microbiology · 2026Review
- Electron transport across the cell envelope via multihemeFrontiers in chemistry · 2025Review
- Article
- Geobacter sulfurreducens metabolism at different donor/acceptor ratios.MicrobiologyOpen · 2022Article
- Prolonged drought imparts lasting compositional changes to the rice root microbiome.Nature plants · 2021Article
- Engineering Cooperation in an Anaerobic Coculture.Applied and environmental microbiology · 2021Article
- Bioelectrochemically enhanced degradation of bisphenol S: mechanistic insights from stable isotope-assisted investigations.iScience · 2021Article
- Comparative proteomics of Geobacter sulfurreducens PCAEnvironmental microbiology · 2021Article
- Production of electricity and reduction of high-fat diet-induced IL-6 by glucose fermentation of Leuconostoc mesenteroides.Biochemical and biophysical research communications · 2020Article
- Article
- Adaptation of Carbon Source Utilization Patterns ofFrontiers in microbiology · 2020Article
- Enhanced Growth of Pilin-Deficient Geobacter sulfurreducens Mutants in Carbon Poor and Electron Donor Limiting Conditions.Microbial ecology · 2019Article
- Expression of Genes and Proteins Involved in Arsenic Respiration and Resistance in Dissimilatory Arsenate-ReducingApplied and environmental microbiology · 2019Article
- Syntrophic Partners Enhance Growth and Respiratory Dehalogenation of Hexachlorobenzene byFrontiers in microbiology · 2018Article
- Comparative metabolomic studies of Alkanivorax xenomutans showing differential power output in a three chambered microbial fuel cell.World journal of microbiology & biotechnology · 2017Article
- Anodic electro-fermentation of 3-hydroxypropionic acid from glycerol by recombinantBiotechnology for biofuels · 2017Article
- Carboxydotrophic growth of Geobacter sulfurreducens.Applied microbiology and biotechnology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGeobacter sulfurreducens is capable of coupling the complete oxidation of organic compounds to iron reduction. The metabolic response of G. sulfurreducens towards variations in electron donors (acetate, hydrogen) and acceptors (Fe(III), fumarate) was investigated via (13)C-based metabolic flux analysis. We examined the (13)C-labeling patterns of proteinogenic amino acids obtained from G. sulfurreducens cultured with (13)C-acetate.
resultsUsing (13)C-based metabolic flux analysis, we observed that donor and acceptor variations gave rise to differences in gluconeogenetic initiation, tricarboxylic acid cycle activity, and amino acid biosynthesis pathways. Culturing G. sulfurreducens cells with Fe(III) as the electron acceptor and acetate as the electron donor resulted in pyruvate as the primary carbon source for gluconeogenesis. When fumarate was provided as the electron acceptor and acetate as the electron donor, the flux analysis suggested that fumarate served as both an electron acceptor and, in conjunction with acetate, a carbon source. Growth on fumarate and acetate resulted in the initiation of gluconeogenesis by phosphoenolpyruvate carboxykinase and a slightly elevated flux through the oxidative tricarboxylic acid cycle as compared to growth with Fe(III) as the electron acceptor. In addition, the direction of net flux between acetyl-CoA and pyruvate was reversed during growth on fumarate relative to Fe(III), while growth in the presence of Fe(III) and acetate which provided hydrogen as an electron donor, resulted in decreased flux through the tricarboxylic acid cycle.
conclusionsWe gained detailed insight into the metabolism of G. sulfurreducens cells under various electron donor/acceptor conditions using (13)C-based metabolic flux analysis. Our results can be used for the development of G. sulfurreducens as a chassis for a variety of applications including bioremediation and renewable biofuel production.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.