ArticleBioTechniques2010
Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes.
Article in BioTechniques, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Article
- Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.Chemphyschem : a European journal of chemical physics and physical chemistry · 2021Article
- Single Molecule FRET: A Powerful Tool to Study Intrinsically Disordered Proteins.Biomolecules · 2018Review
- Near-native, site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis.Scientific reports · 2018Article
- We FRET so You Don't Have To: New Models of the Lipoprotein Lipase Dimer.Biochemistry · 2018Article
- Three tRNAs on the ribosome slow translation elongation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Combining Graphical and Analytical Methods with Molecular Simulations To Analyze Time-Resolved FRET Measurements of Labeled Macromolecules Accurately.The journal of physical chemistry. B · 2017Article
- High Precision FRET at Single-molecule Level for Biomolecule Structure Determination.Journal of visualized experiments : JoVE · 2017Article
- Using Atomic Force Microscopy to Characterize the Conformational Properties of Proteins and Protein-DNA Complexes That Carry Out DNA Repair.Methods in enzymology · 2017Article
- Single-Molecule FRET to Measure Conformational Dynamics of DNA Mismatch Repair Proteins.Methods in enzymology · 2016Article
- Advances in chemical labeling of proteins in living cells.Cell and tissue research · 2015Review
- Eukaryotic Mismatch Repair in Relation to DNA Replication.Annual review of genetics · 2015Review
- Single molecule studies of DNA mismatch repair.DNA repair · 2014Review
- The bright future of single-molecule fluorescence imaging.Current opinion in chemical biology · 2014Review
- Single-molecule FRET experiments with a red-enhanced custom technology SPAD.Proceedings of SPIE--the International Society for Optical Engineering · 2013Article
- Single-molecule nanometry for biological physics.Reports on progress in physics. Physical Society (Great Britain) · 2013Review
- Large conformational changes in MutS during DNA scanning, mismatch recognition and repair signalling.The EMBO journal · 2012Article
- Multicolour single molecule imaging on cells using a supercontinuum source.Biomedical optics express · 2012Article
- Article
- Dynamics of lesion processing by bacterial nucleotide excision repair proteins.Progress in molecular biology and translational science · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
To enable studies of conformational changes within multimolecular complexes, we present a simultaneous, four-color single molecule fluorescence methodology implemented with total internal reflection illumination and camera-based, wide-field detection. We further demonstrate labeling histidine-tagged proteins noncovalently with Tris-nitrilotriacetic acid (Tris-NTA)-conjugated dyes to achieve single molecule detection. We combine these methods to colocalize the mismatch repair protein MutSα on DNA while monitoring MutSα-induced DNA bending using Förster resonance energy transfer (FRET) and to monitor assembly of membrane-tethered SNARE protein complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.