ArticleThe European journal of neuroscience2010
Knockdown of orexin type 1 receptor in rat locus coeruleus increases REM sleep during the dark period.
Article in The European journal of neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- Orexin 2 Receptor Antagonism is Sufficient to Promote NREM and REM Sleep from Mouse to Man.Scientific reports · 2016Trial
- Efficacy and Safety Profile of Suvorexant for the Treatment of Insomnia.Current drug safety · 2026Review
- Anxiety and sleep hygiene among college students, a moderated mediating model.BMC public health · 2025Article
- Cellular Localization of Orexin 1 Receptor in Human Hypothalamus and Morphological Analysis of Neurons Expressing the Receptor.Biomolecules · 2023Article
- Translational approaches to influence sleep and arousal.Brain research bulletin · 2022Review
- REM sleep loss-induced elevated noradrenaline could predispose an individual to psychosomatic disorders: a review focused on proposal for prediction, prevention, and personalized treatment.The EPMA journal · 2020Review
- Orexin Receptor Antagonists as Emerging Treatments for Psychiatric Disorders.Neuroscience bulletin · 2020Review
- Identification of discrete, intermingled hypocretin neuronal populations.The Journal of comparative neurology · 2018Article
- Knockdown of hypocretin attenuates extended access of cocaine self-administration in rats.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Article
- Understanding how discrete populations of hypothalamic neurons orchestrate complicated behavioral states.Frontiers in systems neuroscience · 2015Review
- Orexin A attenuates the sleep-promoting effect of adenosine in the lateral hypothalamus of rats.Neuroscience bulletin · 2014Article
- Animal models of sleep disorders.Comparative medicine · 2013Review
- Knockdown of orexin type 2 receptor in the lateral pontomesencephalic tegmentum of rats increases REM sleep.The European journal of neuroscience · 2013Article
- Medical applications of RNA interference (RNAi).BMC proceedings · 2013Article
- Differential actions of orexin receptors in brainstem cholinergic and monoaminergic neurons revealed by receptor knockouts: implications for orexinergic signaling in arousal and narcolepsy.Frontiers in neuroscience · 2013Article
- Distinct effects of IPSU and suvorexant on mouse sleep architecture.Frontiers in neuroscience · 2013Article
- Mechanism for Hypocretin-mediated sleep-to-wake transitions.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Control of sleep and wakefulness.Physiological reviews · 2012Review
- Article
- Circadian integration of sleep-wake and feeding requires NPY receptor-expressing neurons in the mediobasal hypothalamus.American journal of physiology. Regulatory, integrative and comparative physiology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
The locus coeruleus (LC) regulates sleep/wakefulness and is densely innervated by orexinergic neurons in the lateral hypothalamus. Here we used small interfering RNAs (siRNAs) to test the role of LC orexin type 1 receptor (OxR1) in sleep–wake control. In sleep studies, bilateral OxR1 siRNA injections led to an increase of time spent in rapid eye movement (REM) sleep, which was selective for the dark (active) period, peaked at approximately 30% of control during the second dark period after injection and then disappeared after 4 days. Cataplexy-like episodes were not observed. The percentage time spent in wakefulness and non-REM (NREM) sleep and the power spectral profile of NREM and REM sleep were unaffected. Control animals, injected with scrambled siRNA, had no sleep changes after injection. Quantification of the knockdown revealed that unilateral microinjection of siRNAs targeting OxR1 into the rat LC on two consecutive days induced a 45.5% reduction of OxR1 mRNA in the LC 2 days following the injections when compared with the contralateral side receiving injections of control (scrambled) siRNAs. This reduction disappeared 4 days after injection. Similarly, unilateral injection of OxR1 siRNA into the LC revealed a marked (33.5%) reduction of OxR1 staining 2 days following injections. In contrast, both the mRNA level and immunohistochemical staining for tyrosine hydroxylase were unaffected. The results indicate that a modest knockdown of OxR1 is sufficient to induce observable sleep changes. Moreover, orexin neurons, by acting on OxR1 in the LC, play a role in the diurnal gating of REM sleep.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.