ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2011
Cell autonomous requirement of endocardial Smad4 during atrioventricular cushion development in mouse embryos.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Cardiac EndMT and EpiMT as a developmental continuum: integration of mechanical, metabolic, and epigenetic regulation.Frontiers in cell and developmental biology · 2026Review
- Reassessing endothelial-to-mesenchymal transition in cardiovascular diseases.Nature reviews. Cardiology · 2018Review
- Pdgfrb is a direct regulatory target of TGFβ signaling in atrioventricular cushion mesenchymal cells.PloS one · 2017Article
- BMP-SMAD signalling output is highly regionalized in cardiovascular and lymphatic endothelial networks.BMC developmental biology · 2016Article
- Persistent Noggin arrests cardiomyocyte morphogenesis and results in early in utero lethality.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Article
- Ectopic Noggin in a Population of Nfatc1 Lineage Endocardial Progenitors Induces Embryonic Lethality.Journal of cardiovascular development and disease · 2014Article
- Yap1 is required for endothelial to mesenchymal transition of the atrioventricular cushion.The Journal of biological chemistry · 2014Article
- CHD7 interacts with BMP R-SMADs to epigenetically regulate cardiogenesis in mice.Human molecular genetics · 2014Article
- Myocardial Mycn is essential for mouse ventricular wall morphogenesis.Developmental biology · 2013Article
- Partitioning the heart: mechanisms of cardiac septation and valve development.Development (Cambridge, England) · 2012Review
- Homeobox C9 is not potentially related to congenital heart disease in Chinese patients.Genetic testing and molecular biomarkers · 2012Article
- Transforming growth factor beta signaling in adult cardiovascular diseases and repair.Cell and tissue research · 2012Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Atrioventricular (AV) cushions are the precursors of AV septum and valves. In this study, we examined roles of Smad4 during AV cushion development using a conditional gene inactivation approach. We found that endothelial/endocardial inactivation of Smad4 led to the hypocellular AV cushion defect and that both reduced cell proliferation and increased apoptosis contributed to the defect. Expression of multiple genes critical for cushion development was down-regulated in mutant embryos. In collagen gel assays, the number of mesenchymal cells formed is significantly reduced in mutant AV explants compared to that in control explants, suggesting that the reduction of cushion mesenchyme formation in mutants is unlikely secondary to their gross vasculature abnormalities. Using a previously developed immortal endocardial cell line, we showed that Smad4 is required for BMP signaling- stimulated upregulation of Tbx20 and Gata4. Therefore, our data collectively support the cell-autonomous requirement of endocardial Smad4 in regulating AV cushion development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.