ArticleBiophysical reviews2010
Effects of macromolecular crowding on intracellular diffusion from a single particle perspective.
Article in Biophysical reviews, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Molecular Crowding by Computational Approaches.Sub-cellular biochemistry · 2025Review
- Nonthermal fluctuations accelerate biomolecular motors.Biophysical reviews · 2024Review
- Article
- MIL-CELL: a tool for multi-scale simulation of yeast replication and prion transmission.European biophysics journal : EBJ · 2023Article
- Brownian Motion in Optical Tweezers, a Comparison between MD Simulations and Experimental Data in the Ballistic Regime.Polymers · 2023Article
- Practical considerations for feature assignment in high-speed AFM of live cell membranes.Biophysics and physicobiology · 2022Article
- Growth and Antifungal Resistance of the Pathogenic Yeast,Life (Basel, Switzerland) · 2021Article
- Article
- Unified theoretical description of the kinetics of protein aggregation.Biophysical reviews · 2019Review
- A note of appreciation for Prof. Cristobal dos Remedios on behalf of Biophysical Reviews.Biophysical reviews · 2019Article
- Crowd Control: Effects of Physical Crowding on Cargo Movement in Healthy and Diseased Neurons.Frontiers in cellular neuroscience · 2019Review
- Fractal analysis of lateral movement in biomembranes.European biophysics journal : EBJ · 2018Article
- Do Skeletal Dynamics Mediate Sugar Uptake and Transport in Human Erythrocytes?Biophysical journal · 2018Article
- Ecology of aspergillosis: insights into the pathogenic potency of Aspergillus fumigatus and some other Aspergillus species.Microbial biotechnology · 2017Review
- Theory of Crowding Effects on Bimolecular Reaction Rates.The journal of physical chemistry. B · 2016Article
- Regulating the Membrane Transport Activity and Death of Cells via Electroosmotic Manipulation.Biophysical journal · 2016Article
- A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy.eLife · 2016Article
- From microscopy data to in silico environments for in vivo-oriented simulations.EURASIP journal on bioinformatics & systems biology · 2012Article
- Agent-based simulation of reactions in the crowded and structured intracellular environment: Influence of mobility and location of the reactants.BMC systems biology · 2011Article
- Diffusion in crowded biological environments: applications of Brownian dynamics.BMC biophysics · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compared to biochemical reactions taking place in relatively well-defined aqueous solutions in vitro, the corresponding reactions happening in vivo occur in extremely complex environments containing only 60-70% water by volume, with the remainder consisting of an undefined array of bio-molecules. In a biological setting, such extremely complex and volume-occupied solution environments are termed 'crowded'. Through a range of intermolecular forces and pseudo-forces, this complex background environment may cause biochemical reactions to behave differently to their in vitro counterparts. In this review, we seek to highlight how the complex background environment of the cell can affect the diffusion of substances within it. Engaging the subject from the perspective of a single particle's motion, we place the focus of our review on two areas: (1) experimental procedures for conducting single particle tracking experiments within cells along with methods for extracting information from these experiments; (2) theoretical factors affecting the translational diffusion of single molecules within crowded two-dimensional membrane and three-dimensional solution environments. We conclude by discussing a number of recent publications relating to intracellular diffusion in light of the reviewed material.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.