ArticleNucleic acids research2011
Change of tRNA identity leads to a divergent orthogonal histidyl-tRNA synthetase/tRNAHis pair.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- Genetic Code Expansion: Recent Developments and Emerging Applications.Chemical reviews · 2025Review
- Evolving Escherichia coli to use a tRNA with a non-canonical fold as an adaptor of the genetic code.Nucleic acids research · 2024Article
- Cellular Site-Specific Incorporation of Noncanonical Amino Acids in Synthetic Biology.Chemical reviews · 2024Review
- The tRNA identity landscape for aminoacylation and beyond.Nucleic acids research · 2023Review
- "Not-so-popular" orthogonal pairs in genetic code expansion.Protein science : a publication of the Protein Society · 2023Review
- Evolutionary Adjustment of tRNA Identity Rules in Bacillariophyta for Recognition by an Aminoacyl-tRNA Synthetase Adds a Facet to the Origin of Diatoms.Journal of molecular evolution · 2022Article
- Rational Design of Aptamer-Tagged tRNAs.International journal of molecular sciences · 2020Article
- Review
- A genomically modified Escherichia coli strain carrying an orthogonal E. coli histidyl-tRNA synthetase•tRNABiochimica et biophysica acta. General subjects · 2017Article
- Evolutionary gain of highly divergent tRNA specificities by two isoforms of human histidyl-tRNA synthetase.Cellular and molecular life sciences : CMLS · 2017Article
- Genetic selection for mistranslation rescues a defective co-chaperone in yeast.Nucleic acids research · 2017Article
- Bioinformatic Analysis Reveals Archaeal tRNALife (Basel, Switzerland) · 2017Article
- Repairing tRNA termini: News from the 3' end.RNA biology · 2016Article
- Structural basis for recognition of G-1-containing tRNA by histidyl-tRNA synthetase.Nucleic acids research · 2015Article
- Life without post-transcriptional addition of G-1: two alternatives for tRNAHis identity in Eukarya.RNA (New York, N.Y.) · 2015Article
- From end to end: tRNA editing at 5'- and 3'-terminal positions.International journal of molecular sciences · 2014Review
- Absence of a universal element for tRNAHis identity in Acanthamoeba castellanii.Nucleic acids research · 2013Article
- Suppression of amber codons in Caulobacter crescentus by the orthogonal Escherichia coli histidyl-tRNA synthetase/tRNAHis pair.PloS one · 2013Article
- Doing it in reverse: 3'-to-5' polymerization by the Thg1 superfamily.RNA (New York, N.Y.) · 2012Review
- tRNAHis-guanylyltransferase establishes tRNAHis identity.Nucleic acids research · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mature tRNA(His) has at its 5'-terminus an extra guanylate, designated as G(-1). This is the major recognition element for histidyl-tRNA synthetase (HisRS) to permit acylation of tRNA(His) with histidine. However, it was reported that tRNA(His) of a subgroup of α-proteobacteria, including Caulobacter crescentus, lacks the critical G(-1) residue. Here we show that recombinant C. crescentus HisRS allowed complete histidylation of a C. crescentus tRNA(His) transcript (lacking G(-1)). The addition of G(-1) did not improve aminoacylation by C. crescentus HisRS. However, mutations in the tRNA(His) anticodon caused a drastic loss of in vitro histidylation, and mutations of bases A73 and U72 also reduced charging. Thus, the major recognition elements in C. crescentus tRNA(His) are the anticodon, the discriminator base and U72, which are recognized by the divergent (based on sequence similarity) C. crescentus HisRS. Transplantation of these recognition elements into an Escherichia coli tRNA(His) template, together with addition of base U20a, created a competent substrate for C. crescentus HisRS. These results illustrate how a conserved tRNA recognition pattern changed during evolution. The data also uncovered a divergent orthogonal HisRS/tRNA(His) pair.
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Registered trials
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