ArticleInvestigative ophthalmology & visual science2011
Polarized secretion of PEDF from human embryonic stem cell-derived RPE promotes retinal progenitor cell survival.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 118 citations in OpenAlex.
- Injectable alginate hydrogels improve the effects of subretinal hiPSC-derived RPE cell therapy on retinal degeneration in rats.Materials today. Bio · 2026Article
- Cell therapy for age-related macular degeneration.Mammalian genome : official journal of the International Mammalian Genome Society · 2026Review
- Generation of Induced-Primary Retinal Pigment Epithelium from Human Retinal Organoids.Methods in molecular biology (Clifton, N.J.) · 2025Article
- GSK3 inhibition reduces ECM production and prevents age-related macular degeneration-like pathology.JCI insight · 2024Article
- GSK3 inhibition reduces ECM production and prevents age-related macular degeneration-like pathology.bioRxiv : the preprint server for biology · 2023Article
- Article
- Altered zinc homeostasis in a primary cell culture model of the retinal pigment epithelium.Frontiers in nutrition · 2023Article
- Denuded Descemet's membrane supports human embryonic stem cell-derived retinal pigment epithelial cell culture.PloS one · 2023Article
- PEDF Deletion Induces Senescence and Defects in Phagocytosis in the RPE.International journal of molecular sciences · 2022Article
- Microcarrier-Based Culture of Human Pluripotent Stem-Cell-Derived Retinal Pigmented Epithelium.Bioengineering (Basel, Switzerland) · 2022Article
- Retina stem cells, hopes and obstacles.World journal of stem cells · 2021Review
- Scaffold-Free Retinal Pigment Epithelium Microtissues Exhibit Increased Release of PEDF.International journal of molecular sciences · 2021Article
- Pluripotent stem cell therapy for retinal diseases.Annals of translational medicine · 2021Review
- Cell Replacement Therapy for Retinal and Optic Nerve Diseases: Cell Sources, Clinical Trials and Challenges.Pharmaceutics · 2021Review
- Development of 3D Printed Bruch's Membrane-Mimetic Substance for the Maturation of Retinal Pigment Epithelial Cells.International journal of molecular sciences · 2021Article
- Pigment Epithelium-Derived Factor Peptide Promotes Corneal Nerve Regeneration: An In Vivo and In Vitro Study.Investigative ophthalmology & visual science · 2021Article
- Coculture techniques for modeling retinal development and disease, and enabling regenerative medicine.Stem cells translational medicine · 2020Review
- Partially Differentiated Neuroretinal Cells Promote Maturation of the Retinal Pigment Epithelium.Investigative ophthalmology & visual science · 2020Article
- Lentiviral mediated RPE65 gene transfer in healthy hiPSCs-derived retinal pigment epithelial cells markedly increased RPE65 mRNA, but modestly protein level.Scientific reports · 2020Article
- PEDF peptides promote photoreceptor survival in rd10 retina models.Experimental eye research · 2019Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeHuman embryonic stem cell-derived RPE (hES-RPE) transplantation is a promising therapy for atrophic age-related macular degeneration (AMD); however, future therapeutic approaches may consider co-transplantation of hES-RPE with retinal progenitor cells (RPCs) as a replacement source for lost photoreceptors. The purpose of this study was to determine the effect of polarization of hES-RPE monolayers on their ability to promote survival of RPCs.
methodsThe hES-3 cell line was used for derivation of RPE. Polarization of hES-RPE was achieved by prolonged growth on permeable inserts. RPCs were isolated from 16- to 18-week-gestation human fetal eyes. ELISA was performed to measure pigment epithelium-derived factor (PEDF) levels from conditioned media.
resultsPigmented RPE-like cells appeared as early as 4 weeks in culture and were subcultured at 8 weeks. Differentiated hES-RPE had a normal chromosomal karyotype. Phenotypically polarized hES-RPE cells showed expression of RPE-specific genes. Polarized hES-RPE showed prominent expression of PEDF in apical cytoplasm and a marked increase in secretion of PEDF into the medium compared with nonpolarized culture. RPCs grown in the presence of supernatants from polarized hES-RPE showed enhanced survival, which was ablated by the presence of anti-PEDF antibody.
conclusionshES-3 cells can be differentiated into functionally polarized hES-RPE cells that exhibit characteristics similar to those of native RPE. On polarization, hES-RPE cells secrete high levels of PEDF that can support RPC survival. These experiments suggest that polarization of hES-RPE would be an important feature for promotion of RPC survival in future cell therapy for atrophic AMD.
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