ArticleInvestigative ophthalmology & visual science2011
Age-related gene response of human corneal endothelium to oxidative stress and DNA damage.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Oxidative Stress-Induced Age-Related Bullous Keratopathy in Corneal Endothelial Cells With Lipid Droplets and Vacuoles.Investigative ophthalmology & visual science · 2026Article
- Effect of Physiological Oxygen on Primary Human Corneal Endothelial Cell Cultures.Translational vision science & technology · 2022Article
- A Porcine Organ-Culture Glaucoma Model Mimicking Trabecular Meshwork Damage Using Oxidative Stress.Investigative ophthalmology & visual science · 2021Article
- Cell cycle re-entry and arrest in G2/M phase induces senescence and fibrosis in Fuchs Endothelial Corneal Dystrophy.Free radical biology & medicine · 2021Article
- Antioxidant Defenses in the Human Eye: A Focus on Metallothioneins.Antioxidants (Basel, Switzerland) · 2021Review
- Ultraviolet A light induces DNA damage and estrogen-DNA adducts in Fuchs endothelial corneal dystrophy causing females to be more affected.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Wound-induced polyploidization is driven by Myc and supports tissue repair in the presence of DNA damage.Development (Cambridge, England) · 2019Article
- Article
- A Cell Culture Approach to Optimized Human Corneal Endothelial Cell Function.Investigative ophthalmology & visual science · 2018Article
- Heterogeneity in cancer cells: variation in drug response in different primary and secondary colorectal cancer cell lines in vitro.In vitro cellular & developmental biology. Animal · 2017Article
- Human corneal cell culture models for drug toxicity studies.Drug delivery and translational research · 2016Review
- Oxidative stress and premature senescence in corneal endothelium following penetrating keratoplasty in an animal model.BMC ophthalmology · 2016Article
- A glimpse at the aging eye.NPJ aging and mechanisms of disease · 2016Review
- Aging and radiation: bad companions.Aging cell · 2015Review
- Optimization of immunostaining on flat-mounted human corneas.Molecular vision · 2015Article
- Transcriptome analysis of the human corneal endothelium.Investigative ophthalmology & visual science · 2014Article
- Alive and well? Exploring disease by studying lifespan.Current opinion in genetics & development · 2014Review
- Microarray analysis of cell cycle gene expression in adult human corneal endothelial cells.PloS one · 2014Article
- Common cell biologic and biochemical changes in aging and age-related diseases of the eye: toward new therapeutic approaches to age-related ocular diseases.Investigative ophthalmology & visual science · 2013Review
- Age-related dystrophic changes in corneal endothelium from DNA repair-deficient mice.Aging cell · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeNuclear oxidative DNA damage increases with age in human corneal endothelial cells (HCECs) and contributes to their decreased proliferative capacity. These studies investigated whether HCECs respond to this damage by upregulating their expression of oxidative stress and DNA damage-signaling genes in an age-dependent manner.
methodsHCECs were dissected from the corneas of young (30 years and younger) and older (50 years and older) donors. Total RNA was isolated and reverse-transcribed. Oxidative stress and DNA damage-signaling gene expression were analyzed using commercial PCR-based microarrays. Western blot analyses were conducted on selected proteins to verify the microarray results. Nuclear DNA damage foci were detected in the endothelium of ex vivo corneas by immunostaining for H2AX-Ser139.
resultsFour of 84 genes showed a statistically significant age-related difference in the expression of oxidative stress-related genes; however, Western blot analysis demonstrated an age-related increase in only 2 (cytoglobin and GPX-1) of 11 proteins tested. No age-related differences were detected in the expression of DNA damage-signaling genes. Western blot analysis of seven DNA damage-related proteins verified this finding. Intense nuclear staining of DNA damage foci was observed in nuclei within the central endothelium of older donors. Central endothelium from young donors consistently showed a low level of positive staining.
conclusionsHCECs respond to age-related increases in oxidative nuclear DNA damage by forming DNA damage repair foci; however, they do not vigorously defend against or repair this damage by upregulating the expression of multiple oxidative stress or DNA damage-signaling genes.
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Registered trials
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