ArticleBMC microbiology2010
Development of loop-mediated isothermal amplification (LAMP) assays for rapid detection of Ehrlichia ruminantium.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- Data release: targeted systematic literature search for tick and tick-borne pathogen distributions in six countries in sub-Saharan Africa from 1901 to 2020.Parasites & vectors · 2024Pooled it
- Application of LAMP and TaqMan qPCR for the rapid diagnosis of Anaplasma Capra (an emerging tick-borne zoonotic pathogen) and comparison with Nested-PCR.Veterinary research communications · 2026Article
- Advances in rapid detection technologies for zoonotic diseases: a one health-oriented review.Frontiers in cellular and infection microbiology · 2026Review
- Validation and optimization of the loop-mediated isothermal amplification (LAMP) technique for rapid detection of wheat stripe mosaic virus, a wheat-infecting pathogen.Journal, genetic engineering & biotechnology · 2024Article
- Rapid and sensitive visual detection of avian leukosis virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow immunochromatographic strip assay.Archives of virology · 2024Article
- Distribution and Prevalence ofMicroorganisms · 2023Review
- Visual closed dumbbell-mediated isothermal amplification (CDA) for on-site detection of Rickettsia raoultii.PLoS neglected tropical diseases · 2022Article
- Molecular Prevalence and Identification ofVeterinary medicine international · 2021Article
- Development of a Loop-Mediated Isothermal Amplification (LAMP) Assay Targeting the Citrate Synthase Gene for Detection ofVeterinary sciences · 2020Article
- Genetic diversity of Ehrlichia ruminantium field strains from selected farms in South Africa.The Onderstepoort journal of veterinary research · 2020Article
- The Q-LAMP Method Represents a Valid and Rapid Alternative for the Detection of theInternational journal of molecular sciences · 2019Article
- Detecting amplicons of loop-mediated isothermal amplification.Microbiology and immunology · 2019Review
- Detection of Ehrlichia ruminantium infection in cattle in Cameroon.BMC research notes · 2018Article
- Article
- Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks.Parasites & vectors · 2017Article
- Hinge-initiated Primer-dependent Amplification of Nucleic Acids (HIP) - A New Versatile Isothermal Amplification Method.Scientific reports · 2017Article
- Development and Diagnostic Evaluation of Loop-Mediated Isothermal Amplification Using a New Gene Target for Rapid Detection of Helicobacter pylori.Jundishapur journal of microbiology · 2016Article
- Molecular evidence of spotted fever group rickettsiae and Anaplasmataceae from ticks and stray dogs in Bangladesh.Parasitology research · 2016Article
- Molecular detection of zoonotic tick-borne pathogens from ticks collected from ruminants in four South African provinces.The Journal of veterinary medical science · 2016Article
- Article
Corrections and comments
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Authors and funding
11 authors at 9 institutions in 9 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe rickettsial bacterium Ehrlichia ruminantium is the causative agent of heartwater, a potential zoonotic disease of ruminants transmitted by ticks of the genus Amblyomma. The disease is distributed in nearly all of sub-Saharan Africa and some islands of the Caribbean, from where it threatens the American mainland. This report describes the development of two different loop-mediated isothermal amplification (LAMP) assays for sensitive and specific detection of E. ruminantium.
resultsTwo sets of LAMP primers were designed from the pCS20 and sodB genes. The detection limits for each assay were 10 copies for pCS20 and 5 copies for sodB, which is at least 10 times higher than that of the conventional pCS20 PCR assay. DNA amplification was completed within 60 min. The assays detected 16 different isolates of E. ruminantium from geographically distinct countries as well as two attenuated vaccine isolates. No cross-reaction was observed with genetically related Rickettsiales, including zoonotic Ehrlichia species from the USA. LAMP detected more positive samples than conventional PCR but less than real-time PCR, when tested with field samples collected in sub-Saharan countries.
conclusionsDue to its simplicity and specificity, LAMP has the potential for use in resource-poor settings and also for active screening of E. ruminantium in both heartwater-endemic areas and regions that are at risk of contracting the disease.
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