ArticleMolecular biology reports2011
Lactobacillus plantarum surface layer adhesive protein protects intestinal epithelial cells against tight junction injury induced by enteropathogenic Escherichia coli.
Article in Molecular biology reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Bacterial cell envelope components of gut commensals: effects in host-microbe interaction.Annals of medicine · 2026Review
- The SlpX protein of Lactobacillus acidophilus CICC6074 has anti-inflammatory properties and protects Caco-2 intestinal epithelial cells from TNF-α-induced barrier damage.Journal of the science of food and agriculture · 2026Article
- Surface Layer-Associated Protein on Nanoparticles from Lactobacillus helveticus Enhances the Response to LPS in RAW264.7 Cells.Current microbiology · 2026Article
- Multifunctional antimicrobial effects ofFrontiers in immunology · 2026Article
- Optimistic effects of dual nano-encapsulated probiotics on breeders laying performance, intestinal barrier functions, immunity and resistance against Salmonella Typhimurium challenge.Scientific reports · 2025Article
- Using Postbiotics from Functional Foods for Managing Colorectal Cancer: Mechanisms, Sources, Therapeutic Potential, and Clinical Perspectives.Microorganisms · 2025Review
- Postbiotics in colorectal cancer: intervention mechanisms and perspectives.Frontiers in microbiology · 2024Review
- Mechanisms and applications of probiotics in prevention and treatment of swine diseases.Porcine health management · 2023Review
- Precision Postbiotics and Mental Health: the Management of Post-COVID-19 Complications.Probiotics and antimicrobial proteins · 2022Review
- Emerging role of protein modification in inflammatory bowel disease.Journal of Zhejiang University. Science. B · 2022Review
- Early supplementation with Lactobacillus plantarum in liquid diet modulates intestinal innate immunity through toll-like receptor 4-mediated mitogen-activated protein kinase signaling pathways in young piglets challenged with Escherichia coli K88.Journal of animal science · 2021Article
- Effects of Paeonol and Gastroretention Tablets of Paeonol on Experimental Gastric Ulcers and Intestinal Flora in Rats.Inflammation · 2020Article
- Effects of multi-strain probiotic supplementation on intestinal microbiota, tight junctions, and inflammation in young broiler chickens challenged with Salmonella enterica subsp. enterica.Asian-Australasian journal of animal sciences · 2020Article
- Postbiotics-parabiotics: the new horizons in microbial biotherapy and functional foods.Microbial cell factories · 2020Review
- Surface components and metabolites of probiotics for regulation of intestinal epithelial barrier.Microbial cell factories · 2020Review
- Secretome profiling of Propionibacterium freudenreichii reveals highly variable responses even among the closely related strains.Microbial biotechnology · 2018Article
- Extractable Bacterial Surface Proteins in Probiotic-Host Interaction.Frontiers in microbiology · 2018Review
- Assessing the Intestinal Microbiota in the SHINE Trial.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015Article
- Kefir: a multifaceted fermented dairy product.Probiotics and antimicrobial proteins · 2014Review
- Basic and clinical research on the regulation of the intestinal barrier by Lactobacillus and its active protein components: a review with experience of one center.Molecular biology reports · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactobacillus plantarum (LP) has previously been used for the treatment and prevention of intestinal disorders and disease. However, the role of the LP surface layer adhesive protein (SLAP) in inhibition of epithelial cell disruption is not fully understood. The aim of the present study was to investigate the protective effects of purified SLAP on Caco-2 cells infected with enteropathogenic Escherichia coli (EPEC). The role of ERK in LP-mediated inhibition of tight junction (TJ) injury was also evaluated in order to determine the molecular mechanisms underlying the protective effects of LP in epithelial cells. SLAP was extracted and purified from LP cells using a porcine stomach mucin-Sepharose 4B column. SLAP-mediated inhibition of bacterial adhesion was measured using a competition-based adhesion assay. Expression of TJ-associated proteins, maintenance of TJ structure, and levels of extracellular signal regulated kinase (ERK) and ERK phosphorylation were assessed in SLAP-treated cells by a combination of real-time PCR, western blotting, and immunofluorescence microscopy. Cell permeability was analyzed by measurement of trans-epithelial electrical resistance (TER) and dextran permeability. The effect of SLAP on levels of apoptosis in epithelial cells was assessed by flow cytometry. Results from these experiments revealed that treatment with SLAP decreased the level of adhesion of EPEC to Caco-2 cells. SLAP treatment also enhanced expression of TJ proteins at both the mRNA and protein levels and affected F-actin distribution. Although ERK levels remained unchanged, ERK phosphorylation was increased by SLAP treatment. Caco-2 cells treated with SLAP exhibited increased TER and decreased macromolecular permeability, which was accompanied by a decrease in the level of apoptosis. Together, these results suggest that LP-produced SLAP protects intestinal epithelial cells from EPEC-induced injury, likely through a mechanism involving ERK activation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.