ArticleApplied microbiology and biotechnology2011
Lytic enzyme discovery through multigenomic sequence analysis in Clostridium perfringens.
Article in Applied microbiology and biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Integration of Lysin into Chitosan Nanoparticles for Improving Bacterial Biofilm Inhibition.Applied biochemistry and biotechnology · 2024Article
- Therapeutic potential of bacteriophage endolysins for infections caused by Gram-positive bacteria.Journal of biomedical science · 2023Review
- Novel Lytic Enzyme of Prophage Origin fromInternational journal of molecular sciences · 2021Article
- Phage lysin that specifically eliminates Clostridium botulinum Group I cells.Scientific reports · 2020Article
- Thermophile Lytic Enzyme Fusion Proteins that TargetAntibiotics (Basel, Switzerland) · 2019Article
- Characterization of LysBC17, a Lytic Endopeptidase fromAntibiotics (Basel, Switzerland) · 2019Article
- Validation and Stabilization of a Prophage Lysin of Clostridium perfringens by Using Yeast Surface Display and Coevolutionary Models.Applied and environmental microbiology · 2019Article
- Plant-expressed bacteriophage lysins control pathogenic strains of Clostridium perfringens.Scientific reports · 2018Article
- Article
- Construction of a chimeric lysin Ply187N-V12C with extended lytic activity against staphylococci and streptococci.Microbial biotechnology · 2015Article
- Expression and delivery of an endolysin to combat Clostridium perfringens.Applied microbiology and biotechnology · 2014Article
- Novel chimeric lysin with high-level antimicrobial activity against methicillin-resistant Staphylococcus aureus in vitro and in vivo.Antimicrobial agents and chemotherapy · 2014Article
- Expression of a Clostridium perfringens genome-encoded putative N-acetylmuramoyl-L-alanine amidase as a potential antimicrobial to control the bacterium.Archives of microbiology · 2013Article
- Characteristics of a broad lytic spectrum endolysin from phage BtCS33 of Bacillus thuringiensis.BMC microbiology · 2012Article
- Recombinant Expression of a Putative Amidase Cloned from the Genome of Listeria monocytogenes that Lyses the Bacterium and its Monolayer in Conjunction with a Protease.Probiotics and antimicrobial proteins · 2012Article
- Exploiting what phage have evolved to control gram-positive pathogens.Bacteriophage · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
With their ability to lyse Gram-positive bacteria, phage lytic enzymes (or lysins) have received a great deal of attention as novel anti-infective agents. The number of known genes encoding these peptidoglycan hydrolases has increased markedly in recent years, due in large part to advances in DNA sequencing technology. As the genomes of more and more bacterial species/strains are sequenced, lysin-encoding open reading frames (ORFs) can be readily identified in lysogenized prophage regions. In the current study, we sought to assess lysin diversity for the medically relevant pathogen Clostridium perfringens. The sequenced genomes of nine C. perfringens strains were computationally mined for prophage lysins and lysin-like ORFs, revealing several dozen proteins of various enzymatic classes. Of these lysins, a muramidase from strain ATCC 13124 (termed PlyCM) was chosen for recombinant analysis based on its dissimilarity to previously characterized C. perfringens lysins. Following expression and purification, various biochemical properties of PlyCM were determined in vitro, including pH/salt-dependence and temperature stability. The enzyme exhibited activity at low μg/ml concentrations, a typical value for phage lysins. It was active against 23 of 24 strains of C. perfringens tested, with virtually no activity against other clostridial or non-clostridial species. Overall, PlyCM shows potential for development as an enzybiotic agent, demonstrating how expanding genomic databases can serve as rich pools for biotechnologically relevant proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.