ArticlePloS one2010
Cell-cycle inhibition by Helicobacter pylori L-asparaginase.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Insights into diversity and L-asparaginase activity of fungal endophytes associated with medicinal plant Grewia hirsuta.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Article
- Microbial L-asparaginase as a promising enzyme for treatment of various cancers.Applied microbiology and biotechnology · 2022Review
- Article
- Expanding the bio-catalysis scope and applied perspectives of nanocarrier immobilized asparaginases.3 Biotech · 2021Review
- The Type 3 Deiodinase Is a Critical Modulator of Thyroid Hormone Sensitivity in the Fetal Brain.Frontiers in neuroscience · 2021Article
- Recent Strategies and Applications for l-Asparaginase Confinement.Molecules (Basel, Switzerland) · 2020Review
- Deciphering Additional Roles for the EF-Tu, l-Asparaginase II and OmpT Proteins of Shiga Toxin-ProducingMicroorganisms · 2020Article
- Fecal Metaproteomic Analysis Reveals Unique Changes of the Gut Microbiome Functions After Consumption of SourdoughFrontiers in microbiology · 2019Article
- L-Asparaginase of Leishmania donovani: Metabolic target and its role in Amphotericin B resistance.International journal for parasitology. Drugs and drug resistance · 2017Article
- Article
- In silico analysis, molecular cloning, expression and characterization of l-asparaginase gene from3 Biotech · 2017Article
- Helicobacter pylori-induced premature senescence of extragastric cells may contribute to chronic skin diseases.Biogerontology · 2017Article
- Elucidation of the Metabolic Network of Helicobacter pylori J99 and Malaysian Clinical Strains by Phenotype Microarray.Helicobacter · 2017Article
- Impact of Helicobacter pylori on the healing process of the gastric barrier.World journal of gastroenterology · 2016Article
- Recombinant L-Asparaginase from Zymomonas mobilis: A Potential New Antileukemic Agent Produced in Escherichia coli.PloS one · 2016Article
- Leishmania infantum Asparagine Synthetase A Is Dispensable for Parasites Survival and Infectivity.PLoS neglected tropical diseases · 2016Article
- Suppression of cell division-associated genes by Helicobacter pylori attenuates proliferation of RAW264.7 monocytic macrophage cells.Scientific reports · 2015Article
- Tackling Critical Catalytic Residues in Helicobacter pylori L-Asparaginase.Biomolecules · 2015Article
- Engineering of Helicobacter pylori L-asparaginase: characterization of two functionally distinct groups of mutants.PloS one · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
21 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Helicobacter pylori (H. pylori) is a major human pathogen causing chronic gastritis, peptic ulcer, gastric cancer, and mucosa-associated lymphoid tissue lymphoma. One of the mechanisms whereby it induces damage depends on its interference with proliferation of host tissues. We here describe the discovery of a novel bacterial factor able to inhibit the cell-cycle of exposed cells, both of gastric and non-gastric origin. An integrated approach was adopted to isolate and characterise the molecule from the bacterial culture filtrate produced in a protein-free medium: size-exclusion chromatography, non-reducing gel electrophoresis, mass spectrometry, mutant analysis, recombinant protein expression and enzymatic assays. L-asparaginase was identified as the factor responsible for cell-cycle inhibition of fibroblasts and gastric cell lines. Its effect on cell-cycle was confirmed by inhibitors, a knockout strain and the action of recombinant L-asparaginase on cell lines. Interference with cell-cycle in vitro depended on cell genotype and was related to the expression levels of the concurrent enzyme asparagine synthetase. Bacterial subcellular distribution of L-asparaginase was also analysed along with its immunogenicity. H. pylori L-asparaginase is a novel antigen that functions as a cell-cycle inhibitor of fibroblasts and gastric cell lines. We give evidence supporting a role in the pathogenesis of H. pylori-related diseases and discuss its potential diagnostic application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.