ArticleNature protocols2010
Simultaneous analysis of relative protein expression levels across multiple samples using iTRAQ isobaric tags with 2D nano LC-MS/MS.
Article in Nature protocols, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.
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Who cites it
116 citing papers in PubMed.
- Identification of Candidate Biomarkers in Malignant Ascites from Patients with Hepatocellular Carcinoma by iTRAQ-Based Quantitative Proteomic Analysis.BioMed research international · 2018Trial
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- EVC protein regulates Sonic hedgehog signaling during human intervertebral disc development and degeneration.iScience · 2026Article
- Density-mediated freshwater plastisphere microbiomes preferentially degrade conventional rather than biodegradable microplastics.The ISME journal · 2026Article
- iTRAQ-Based Proteomic Approach to Study Stress Memory Responses in Plants.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Proteomics Approaches Used to Investigate Age Related Macular Degeneration.Advances in experimental medicine and biology · 2026Review
- CLN7 protein functions at the interface between endolysosomes and stress granules to promote cell survival.Cell death & disease · 2025Article
- The Proteomic and Peptidomic Response of Wheat (Plants (Basel, Switzerland) · 2025Article
- Comparative Label-Based Proteomics of Venoms fromProteomes · 2025Article
- Cracking the Kinase Code: Urinary Biomarkers as Early Alarms for AAA Rupture-A Pilot Study.Journal of clinical medicine · 2025Article
- Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome.Clinical and translational medicine · 2025Article
- Article
- Dysregulation of extracellular vesicle protein cargo in female myalgic encephalomyelitis/chronic fatigue syndrome cases and sedentary controls in response to maximal exercise.Journal of extracellular vesicles · 2024Article
- Specific functions of single pistil S-RNases in S-gene homozygous Pyrus germplasm.BMC plant biology · 2023Article
- Vinculin Identified as a Potential Biomarker in Hand-Arm Vibration Syndrome Based on iTRAQ and LC-MS/MS-Based Proteomic Analysis.Journal of proteome research · 2023Article
- Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma.Disease markers · 2023Article
- Cinnamaldehyde ResistMolecules (Basel, Switzerland) · 2022Article
- Heterozygous LRP1 deficiency causes developmental dysplasia of the hip by impairing triradiate chondrocytes differentiation due to inhibition of autophagy.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
56 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
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Abstract
In this paper, we describe the use of iTRAQ (isobaric Tags for Relative and Absolute Quantitation) tags for comparison of protein expression levels between multiple samples. These tags label all peptides in a protein digest before labeled samples are pooled, fractionated and analyzed using mass spectrometry (MS). As the tags are isobaric, the intensity of each peak is the sum of the intensity of this peptide from all samples, providing a moderate enhancement in sensitivity. On peptide fragmentation, amino-acid sequence ions also show this summed intensity, providing a sensitivity enhancement. However, the distinct distribution of isotopes in the tags is such that, on further fragmentation, a tag-specific reporter ion is released. The relative intensities of these ions represent the relative amount of peptide in the analytes. Integration of the relative quantification data for the peptides allows relative quantification of the protein. This protocol discusses the rationale behind design, optimization and performance of experiments, comparing protein samples using iTRAQ chemistries combined with strong cation exchange chromatographic fractionation and MS.
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