ArticleJournal of clinical microbiology2011
Comparison of three molecular techniques for typing Pseudomonas aeruginosa isolates in sputum samples from patients with cystic fibrosis.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed.
- Culture and Adaptation of Pseudomonas aeruginosa During Early Infection in Children With Cystic Fibrosis.The Journal of infectious diseases · 2026Article
- Genetic diversity of Pseudomonas aeruginosa isolated from clinical samples with ISSR molecular marker in a tertiary care teaching hospital.Scientific reports · 2026Article
- Molecular Epidemiology ofAntibiotics (Basel, Switzerland) · 2024Review
- Emergence of Carbapenem-Resistant ST244, ST292, and ST2446Infection and drug resistance · 2022Article
- A population-level strain genotyping method to study pathogen strain dynamics in human infections.JCI insight · 2021Article
- Tracing Mastitis Pathogens-Epidemiological Investigations of aAnimals : an open access journal from MDPI · 2021Article
- Why? - Successful Pseudomonas aeruginosa clones with a focus on clone C.FEMS microbiology reviews · 2020Review
- Prevalence of qnrVC Genes in Pseudomonas aeruginosa Clinical Isolates from Guangdong, China.Current microbiology · 2020Article
- A Core Genome Multilocus Sequence Typing Scheme forFrontiers in microbiology · 2020Article
- Antibiotic ResistantFoods (Basel, Switzerland) · 2019Review
- Epidemiology and molecular characterization of the antimicrobial resistance ofCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2019Article
- Endemic dissemination of different sequence types of carbapenem-resistantInfection and drug resistance · 2019Article
- Epidemiology, Biology, and Impact of Clonal Pseudomonas aeruginosa Infections in Cystic Fibrosis.Clinical microbiology reviews · 2018Review
- "Pathogen Eradication" and "Emerging Pathogens": Difficult Definitions in Cystic Fibrosis.Journal of clinical microbiology · 2018Article
- Metabolic Phenotyping and Strain Characterisation of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients Using Rapid Evaporative Ionisation Mass Spectrometry.Scientific reports · 2018Article
- Features of urinary Escherichia coli isolated from children with complicated and uncomplicated urinary tract infections in Mexico.PloS one · 2018Article
- Isolation, Identification, Prevalence, and Genetic Diversity ofFrontiers in microbiology · 2018Article
- Genomics and Susceptibility Profiles of Extensively Drug-Resistant Pseudomonas aeruginosa Isolates from Spain.Antimicrobial agents and chemotherapy · 2017Article
- Evolution of the Pseudomonas aeruginosa mutational resistome in an international Cystic Fibrosis clone.Scientific reports · 2017Article
- A prospective survey ofAntimicrobial resistance and infection control · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Monitoring the emergence and transmission of Pseudomonas aeruginosa strains among cystic fibrosis (CF) patients is important for infection control in CF centers internationally. A recently developed multilocus sequence typing (MLST) scheme is used for epidemiologic analyses of P. aeruginosa outbreaks; however, little is known about its suitability for isolates from CF patients compared with that of pulsed-field gel electrophoresis (PFGE) and enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). As part of a prevalence study of P. aeruginosa strains in Australian CF clinics, we compared the discriminatory power and concordance of ERIC-PCR, PFGE, and MLST among 93 CF sputum and 11 control P. aeruginosa isolates. PFGE and MLST analyses were also performed on 30 paired isolates collected 85 to 354 days apart from 30 patients attending two CF centers separated by 3,600 kilometers in order to detect within-host evolution. Each of the three methods displayed high levels of concordance and discrimination; however, overall lower discrimination was seen with ERIC-PCR than with MLST and PFGE. Analysis of the 50 ERIC-PCR types yielded 54 PFGE types, which were related by ≤ 6 band differences, and 59 sequence types, which were classified into 7 BURST groups and 42 singletons. MLST also proved useful for detecting novel and known strains and for inferring relatedness among unique PFGE types. However, 47% of the paired isolates produced PFGE patterns that within 1 year differed by one to five bands, whereas with MLST all paired isolates remained identical. MLST thus represents a categorical analysis tool with resolving power similar to that of PFGE for typing P. aeruginosa. Its focus on highly conserved housekeeping genes is particularly suited for long-term clinical monitoring and detecting novel strains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.