ArticleJournal of the Royal Society, Interface2011
Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification.
Article in Journal of the Royal Society, Interface, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 35 citations in OpenAlex.
- The Pre-Polarization and Concentration of Cells near Micro-Electrodes Using AC Electric Fields Enhances the Electrical Cell Lysis in a Sessile Drop.Biosensors · 2025Article
- A low-voltage alternant direct current electroporation chip for ultrafast releasing the genome DNA of Helicobacter pylori bacterium.Mikrochimica acta · 2024Article
- Separation, Characterization, and Handling of Microalgae by Dielectrophoresis.Microorganisms · 2020Review
- Electrochemical cell lysis of gram-positive and gram-negative bacteria: DNA extraction from environmental water samples.Electrochimica acta · 2020Article
- Molecular harvesting with electroporation for tissue profiling.Scientific reports · 2019Article
- Microfluidic techniques for enhancing biofuel and biorefinery industry based on microalgae.Biotechnology for biofuels · 2019Article
- Microfluidic Sample Preparation for Single Cell Analysis.Analytical chemistry · 2016Review
- Microfluidic electroporation for cellular analysis and delivery.Lab on a chip · 2013Review
- Flow process for electroextraction of total proteins from microalgae.The Journal of membrane biology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe here a simple device for dielectrophoretic concentration of marine microalga Karenia brevis non-motile cells, followed by electric field-mediated lysis for RNA extraction. The lysate was purified using magnetic beads and pure RNA extracted. RNA quality was assessed off-chip by nucleic acid sequence-based amplification and the optimum conditions for lysis were determined. This procedure will form part of an integrated microfluidic system that is being developed with sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time quantitative RNA detection. The integrated system and its components could be used for a large range of applications including in situ harmful algal bloom detection, transcriptomics and point-of-care diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.