ArticleThe Journal of biological chemistry2011
Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (NAB) 3 protein.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- The Nrd1-Nab3-Sen1 transcription termination complex from a structural perspective.Biochemical Society transactions · 2023Review
- Assessing the in vitro Binding Affinity of Protein-RNA Interactions Using an RNA Pull-down Technique.Bio-protocol · 2022Article
- Structural basis of Nrd1-Nab3 heterodimerization.Life science alliance · 2022Article
- Modulated termination of non-coding transcription partakes in the regulation of gene expression.Nucleic acids research · 2022Article
- Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro.Journal of molecular biology · 2020Article
- A non-canonical role for the EDC4 decapping factor in regulating MARF1-mediated mRNA decay.eLife · 2020Article
- A crucial RNA-binding lysine residue in the Nab3 RRM domain undergoes SET1 and SET3-responsive methylation.Nucleic acids research · 2020Article
- Termination of non-coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD-mimicking region in Sen1.The EMBO journal · 2020Article
- RNA Polymerase II CTD phosphatase Rtr1 fine-tunes transcription termination.PLoS genetics · 2020Article
- The structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition.Nucleic acids research · 2017Article
- Structural insight into recognition of phosphorylated threonine-4 of RNA polymerase II C-terminal domain by Rtt103p.EMBO reports · 2017Article
- Biochemical characterization of the helicase Sen1 provides new insights into the mechanisms of non-coding transcription termination.Nucleic acids research · 2017Article
- Evidence for cooperative tandem binding of hnRNP C RRMs in mRNA processing.RNA (New York, N.Y.) · 2015Article
- RBM7 subunit of the NEXT complex binds U-rich sequences and targets 3'-end extended forms of snRNAs.Nucleic acids research · 2015Article
- Nab3 facilitates the function of the TRAMP complex in RNA processing via recruitment of Rrp6 independent of Nrd1.PLoS genetics · 2015Article
- Termination of Transcription of Short Noncoding RNAs by RNA Polymerase II.Annual review of biochemistry · 2015Review
- Molecular basis for coordinating transcription termination with noncoding RNA degradation.Molecular cell · 2014Article
- Structure and semi-sequence-specific RNA binding of Nrd1.Nucleic acids research · 2014Article
- PAR-CLIP data indicate that Nrd1-Nab3-dependent transcription termination regulates expression of hundreds of protein coding genes in yeast.Genome biology · 2014Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Non-coding RNA polymerase II transcripts are processed by the poly(A)-independent termination pathway that requires the Nrd1 complex. The Nrd1 complex includes two RNA-binding proteins, the nuclear polyadenylated RNA-binding (Nab) 3 and the nuclear pre-mRNA down-regulation (Nrd) 1 that bind their specific termination elements. Here we report the solution structure of the RNA-recognition motif (RRM) of Nab3 in complex with a UCUU oligonucleotide, representing the Nab3 termination element. The structure shows that the first three nucleotides of UCUU are accommodated on the β-sheet surface of Nab3 RRM, but reveals a sequence-specific recognition only for the central cytidine and uridine. The specific contacts we identified are important for binding affinity in vitro as well as for yeast viability. Furthermore, we show that both RNA-binding motifs of Nab3 and Nrd1 alone bind their termination elements with a weak affinity. Interestingly, when Nab3 and Nrd1 form a heterodimer, the affinity to RNA is significantly increased due to the cooperative binding. These findings are in accordance with the model of their function in the poly(A) independent termination, in which binding to the combined and/or repetitive termination elements elicits efficient termination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.