ArticleTobacco induced diseases2010
Half a pack of cigarettes a day more than doubles DNA breaks in circulating leukocytes.
Article in Tobacco induced diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Effects of oral supplementation with whey protein concentrate and its hydrolysates on blood cholesterol levels and oxidative DNA damage in South Korean male smokers.Clinical nutrition research · 2026Article
- Article
- The discovery of the Flammer syndrome: a historical and personal perspective.The EPMA journal · 2017Review
- Nutritional recommendations for individuals with Flammer syndrome.The EPMA journal · 2017Review
- Comet assay as an indirect measure of systemic oxidative stress.Journal of visualized experiments : JoVE · 2015Article
- In Vivo Autofluorescence Spectroscopic Study and Evaluation of DNA Damage By Comet Assay in Smokers.Journal of clinical and diagnostic research : JCDR · 2015Article
- Retinal venous pressure in chronic smokers.The EPMA journal · 2015Article
- Menthol-enhanced cytotoxicity of cigarette smoke demonstrated in two bioassay models.Tobacco induced diseases · 2013Article
- Anti-inflammatory effects of nicotine in obesity and ulcerative colitis.Journal of translational medicine · 2011Review
- Lethal impacts of cigarette smoke in cultured tobacco cells.Tobacco induced diseases · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe mechanisms by which smoking induces damage is not known for all diseases. One mechanism believed to play a role is oxidative stress. Oxidative stress leads to cellular damage including DNA damage, particularly DNA breaks. We conducted this study to test the hypothesis that smokers have increased DNA breaks in their circulating leukocytes.
methodsA comparative quantification of single-stranded DNA breaks was performed by comet assay analysis in the circulating leukocytes of ten healthy smokers (average smoking rate: half a pack a day, range: 9-12 cigarettes a day) and ten age and sex matched healthy non-smokers. DNA breaks lead to smaller pieces of DNA, which migrate out of the nucleus forming a tail during gel-electrophoresis. Damage of an individual cell was quantified by the parameters tail moment and olive moment.
resultsSmoking had a clear effect on both study parameters (tail and olive moment). Smokers had more than double the amount of ss-DNA breaks in their circulating leukocytes than non-smokers [tail moment: 0·75 AU [smokers] compared to 0·2 AU [non-smokers]; olive moment: 0·85 AU [smokers] compared to 0·3 AU [non-smokers]; both p < 0·001].
conclusionSmoking half a pack a day interferes with DNA integrity. One potential explanation for the enhanced DNA breaks in smokers is oxidative stress.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.