ArticlePloS one2010
Expression and membrane topology of Anopheles gambiae odorant receptors in lepidopteran insect cells.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 67 citations in OpenAlex.
- Genome-wide identification of olfactory receptor and odorant-binding protein gene families and their roles in Heliothine chemosensory evolution.Insect molecular biology · 2026Article
- The chemosensory world of mosquitoes: olfactory receptors and their role in blocking mosquito-borne disease transmission.Parasites & vectors · 2025Review
- Transcuticular calcium imaging as a tool for the functional study of insect odorant receptors.Frontiers in molecular neuroscience · 2023Article
- Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae.Cell reports · 2022Article
- Screening of olfactory genes related to blood-feeding behaviors in Culex pipiens quinquefasciatus and Culex pipiens molestus by transcriptome analysis.PLoS neglected tropical diseases · 2022Article
- Targeting Insect OlfactionFrontiers in cellular neuroscience · 2022Article
- Optimization of Insect Odorant Receptor Trafficking and Functional Expression Via Transient Transfection in HEK293 Cells.Chemical senses · 2019Article
- Evolutionary Analysis of the Highly Conserved Insect Odorant Coreceptor (Orco) Revealed a Positive Selection Mode, Implying Functional Flexibility.Journal of insect science (Online) · 2018Article
- Mass Spectrometry-Based Screening Platform Reveals Orco Interactome inMolecules and cells · 2018Article
- Access to the odor world: olfactory receptors and their role for signal transduction in insects.Cellular and molecular life sciences : CMLS · 2018Review
- Odorant Receptors and Odorant-Binding Proteins as Insect Pest Control Targets: A Comparative Analysis.Frontiers in physiology · 2018Review
- Expressional and functional interactions of twoPeerJ · 2018Article
- Insect Pheromone Receptors - Key Elements in Sensing Intraspecific Chemical Signals.Frontiers in cellular neuroscience · 2018Review
- Inhibition of insect olfactory behavior by an airborne antagonist of the insect odorant receptor co-receptor subunit.PloS one · 2017Article
- Comparison of research methods for functional characterization of insect olfactory receptors.Scientific reports · 2016Article
- Silencing the Olfactory Co-Receptor RferOrco Reduces the Response to Pheromones in the Red Palm Weevil, Rhynchophorus ferrugineus.PloS one · 2016Article
- Positive Allosteric Modulation of Insect Olfactory Receptor Function by ORco Agonists.Frontiers in cellular neuroscience · 2016Article
- Inhibition of Anopheles gambiae odorant receptor function by mosquito repellents.The Journal of biological chemistry · 2015Article
- Amino acid coevolution reveals three-dimensional structure and functional domains of insect odorant receptors.Nature communications · 2015Article
- A determinant of odorant specificity is located at the extracellular loop 2-transmembrane domain 4 interface of an Anopheles gambiae odorant receptor subunit.Chemical senses · 2014Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A lepidopteran insect cell-based expression system has been employed to express three Anopheles gambiae odorant receptors (ORs), OR1 and OR2, which respond to components of human sweat, and OR7, the ortholog of Drosophila's OR83b, the heteromerization partner of all functional ORs in that system. With the aid of epitope tagging and specific antibodies, efficient expression of all ORs was demonstrated and intrinsic properties of the proteins were revealed. Moreover, analysis of the orientation of OR1 and OR2 on the cellular plasma membrane through the use of a novel 'topology screen' assay and FACS analysis demonstrates that, as was recently reported for the ORs in Drosophila melanogaster, mosquito ORs also have a topology different than their mammalian counterparts with their N-terminal ends located in the cytoplasm and their C-terminal ends facing outside the cell. These results set the stage for the production of mosquito ORs in quantities that should permit their detailed biochemical and structural characterization and the exploration of their functional properties.
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