ArticleNature communications2010
A plasmid-based multigene expression system for mammalian cells.
Article in Nature communications, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The trial behind it
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Who cites it
32 citing papers in PubMed, 72 citations in OpenAlex.
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- Transport of CLCA2 to the nucleus by extracellular vesicles controls keratinocyte survival and migration.Journal of extracellular vesicles · 2024Article
- Direct interrogation of context-dependent GPCR activity with a universal biosensor platform.Cell · 2024Article
- Direct interrogation of context-dependent GPCR activity with a universal biosensor platform.bioRxiv : the preprint server for biology · 2024Article
- Systematic comparison of nonviral gene delivery strategies for efficient co-expression of two transgenes in human mesenchymal stem cells.Journal of biological engineering · 2023Article
- Construction of a bioluminescence-based assay for bitter taste receptors (TAS2Rs).Scientific reports · 2022Article
- Expression of the Heterotrimeric GP2/GP3/GP4 Spike of an Arterivirus in Mammalian Cells.Viruses · 2022Article
- Exploring the signaling space of a GPCR using bivalent ligands with a rigid oligoproline backbone.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Control of Multigene Expression Stoichiometry in Mammalian Cells Using Synthetic Promoters.ACS synthetic biology · 2021Article
- New Insights into Arrestin Recruitment to GPCRs.International journal of molecular sciences · 2020Article
- Light-Inducible Generation of Membrane Curvature in Live Cells with Engineered BAR Domain Proteins.ACS synthetic biology · 2020Article
- Pharmacological inhibition of mTORC1 increases CCKBR-specific tumor uptake of radiolabeled minigastrin analogue [Theranostics · 2020Article
- Synthetic energy sensor AMPfret deciphers adenylate-dependent AMPK activation mechanism.Nature communications · 2019Article
- MultiBacMam Bimolecular Fluorescence Complementation (BiFC) tool-kit identifies new small-molecule inhibitors of the CDK5-p25 protein-protein interaction (PPI).Scientific reports · 2018Article
- Interaction between theMolecular biology of the cell · 2018Article
- A large-scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand-binding domains of taste receptor.Protein science : a publication of the Protein Society · 2017Article
- Highly efficient baculovirus-mediated multigene delivery in primary cells.Nature communications · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The introduction of heterologous genetic information, particularly of multiple genes, into mammalian cells is a key technology in contemporary experimental biological research. The coexpression of fluorescently tagged sensors is required to simultaneously analyse multiple parameters in living cells and the coexpression of several proteins is necessary to manipulate cell fate in stem cell biology. Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. In this paper we describe MultiLabel, a novel and highly efficient modular plasmid-based eukaryotic expression system. Independent expression vectors are assembled by a Cre/LoxP reaction into a plasmid with multiple expression cassettes. MultiLabel enables rapid construction of multigene expression vectors for the single-step creation of transiently or stably transfected mammalian cells.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.