ArticleThe Plant cell2010
Structural and metabolic transitions of C4 leaf development and differentiation defined by microscopy and quantitative proteomics in maize.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers, 1 of them a synthesis that pooled it.
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116 citing papers in PubMed, 1 synthesis or guideline pooled it, 229 citations in OpenAlex.
- Freeze-quenched maize mesophyll and bundle sheath separation uncovers bias in previous tissue-specific RNA-Seq data.Journal of experimental botany · 2017Pooled it
- Faster relaxation of nonphotochemical quenching in C4 than in C3 species.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Systems analysis of leaf anatomy and transcriptome using unsupervised machine learning provides insight on photosynthesis development and evolution in non-model grasses.Plant direct · 2026Article
- Bundle sheath cell-specific expression of chloroplast genes encoding subunits of the NADH dehydrogenase-like complex in maize.The Plant journal : for cell and molecular biology · 2025Article
- Biochemical and structural differences between CScientific reports · 2025Article
- C4 plants respond to phosphate starvation differently than C3 plants.Plant physiology · 2025Article
- In vivo two-photon FLIM resolves photosynthetic properties of maize bundle sheath cells.Photosynthesis research · 2025Article
- Leaf transcriptomes from C3, C3-C4 intermediate, and C4Neurachne species give insights into C4 photosynthesis evolution.Plant physiology · 2024Article
- Novel resources to investigate leaf plasmodesmata formation in CThe Plant journal : for cell and molecular biology · 2024Article
- Plastid retrograde signaling: A developmental perspective.The Plant cell · 2024Review
- Article
- C4 Phosphoenolpyruvate Carboxylase: Evolution and transcriptional regulation.Genetics and molecular biology · 2024Article
- Review
- Article
- Comparative transcriptomics reveals the role of altered energy metabolism in the establishment of single-cell CFrontiers in plant science · 2023Article
- Correlated retrograde and developmental regulons implicate multiple retrograde signals as coordinators of chloroplast development in maize.The Plant cell · 2022Article
- Cotton (International journal of molecular sciences · 2022Article
- Past accomplishments and future challenges of the multi-omics characterization of leaf growth.Plant physiology · 2022Review
- Accelerated remodeling of the mesophyll-bundle sheath interface in the maize C4 cycle mutant leaves.Scientific reports · 2022Article
- Article
56 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C(4) grasses, such as maize (Zea mays), have high photosynthetic efficiency through combined biochemical and structural adaptations. C(4) photosynthesis is established along the developmental axis of the leaf blade, leading from an undifferentiated leaf base just above the ligule into highly specialized mesophyll cells (MCs) and bundle sheath cells (BSCs) at the tip. To resolve the kinetics of maize leaf development and C(4) differentiation and to obtain a systems-level understanding of maize leaf formation, the accumulation profiles of proteomes of the leaf and the isolated BSCs with their vascular bundle along the developmental gradient were determined using large-scale mass spectrometry. This was complemented by extensive qualitative and quantitative microscopy analysis of structural features (e.g., Kranz anatomy, plasmodesmata, cell wall, and organelles). More than 4300 proteins were identified and functionally annotated. Developmental protein accumulation profiles and hierarchical cluster analysis then determined the kinetics of organelle biogenesis, formation of cellular structures, metabolism, and coexpression patterns. Two main expression clusters were observed, each divided in subclusters, suggesting that a limited number of developmental regulatory networks organize concerted protein accumulation along the leaf gradient. The coexpression with BSC and MC markers provided strong candidates for further analysis of C(4) specialization, in particular transporters and biogenesis factors. Based on the integrated information, we describe five developmental transitions that provide a conceptual and practical template for further analysis. An online protein expression viewer is provided through the Plant Proteome Database.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.