ArticleBiophysical journal2010
Nonlinear optical imaging and Raman microspectrometry of the cell nucleus throughout the cell cycle.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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Who cites it
40 citing papers in PubMed, 94 citations in OpenAlex.
- Advancing Label-Free Imaging Through CARS Microscopy: From Signal Formation to Biological Interpretation.International journal of molecular sciences · 2026Review
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Demixing and Analysis of Complex Biological Raman Hyperspectra Based on Peak Fitting, Amplitude Trend Clustering, and Spectrum Reconstruction.Applied spectroscopy · 2025Article
- Mitochondrial Dysfunction and Apoptosis in Brain Microvascular Endothelial Cells Following Blast Traumatic Brain Injury.Cellular and molecular neurobiology · 2023Article
- Viewing life without labels under optical microscopes.Communications biology · 2023Review
- Label-Free Cytometric Evaluation of Mitosis via Stimulated Raman Scattering Microscopy and Spectral Phasor Analysis.Analytical chemistry · 2023Article
- Non-invasive monitoring of microbial triterpenoid production using nonlinear microscopy techniques.Frontiers in bioengineering and biotechnology · 2023Article
- Direct imaging of intracellular RNA, DNA, and liquid-liquid phase separated membraneless organelles with Raman microspectroscopy.Communications biology · 2022Article
- Surfactancy in a tadpole model of proteins.Journal of the Royal Society, Interface · 2022Article
- Advances in measuring cancer cell metabolism with subcellular resolution.Nature methods · 2022Review
- CARS Imaging Advances Early Diagnosis of Cardiac Manifestation of Fabry Disease.International journal of molecular sciences · 2022Article
- Mitochondrial Dysfunction: A Prelude to Neuropathogenesis of SARS-CoV-2.ACS chemical neuroscience · 2022Article
- Raman and fluorescence micro-spectroscopy applied for the monitoring of sunitinib-loaded porous silicon nanocontainers in cardiac cells.Frontiers in pharmacology · 2022Article
- A Single-Organelle Optical Omics Platform for Cell Science and Biomarker Discovery.Analytical chemistry · 2021Article
- Modulation of DNA Damage Response by Sphingolipid Signaling: An Interplay that Shapes Cell Fate.International journal of molecular sciences · 2020Review
- Multiplex coherent anti-Stokes Raman scattering highlights state of chromatin condensation in CH region.Scientific reports · 2019Article
- Inkjet-printed micro-calibration standards for ultraquantitative Raman spectral cytometry.The Analyst · 2019Article
- Three-Dimensional Cellular Raman Analysis: Evidence of Highly Ordered Lipids Within Cell Nuclei.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2018Article
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Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fundamental understanding of cellular processes at molecular level is of considerable importance in cell biology as well as in biomedical disciplines for early diagnosis of infection and cancer diseases, and for developing new molecular medicine-based therapies. Modern biophotonics offers exclusive capabilities to obtain information on molecular composition, organization, and dynamics in a cell by utilizing a combination of optical spectroscopy and optical imaging. We introduce here a combination of Raman microspectrometry, together with coherent anti-Stokes Raman scattering (CARS) and two-photon excited fluorescence (TPEF) nonlinear optical microscopy, to study macromolecular organization of the nucleus throughout the cell cycle. Site-specific concentrations of proteins, DNA, RNA, and lipids were determined in nucleoli, nucleoplasmic transcription sites, nuclear speckles, constitutive heterochromatin domains, mitotic chromosomes, and extrachromosomal regions of mitotic cells by quantitative confocal Raman microspectrometry. A surprising finding, obtained in our study, is that the local concentration of proteins does not increase during DNA compaction. We also demonstrate that postmitotic DNA decondensation is a gradual process, continuing for several hours. The quantitative Raman spectroscopic analysis was corroborated with CARS/TPEF multimodal imaging to visualize the distribution of protein, DNA, RNA, and lipid macromolecules throughout the cell cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.