ArticleBiophysical journal2010
Effect of sphingomyelin headgroup size on molecular properties and interactions with cholesterol.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 76 citations in OpenAlex.
- Structural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains.Biophysical journal · 2023Article
- Sea Anemones, Actinoporins, and Cholesterol.International journal of molecular sciences · 2022Review
- A Study of the Interaction of a New Benzimidazole Schiff Base with Synthetic and Simulated Membrane Models of Bacterial and Mammalian Membranes.Membranes · 2021Article
- Oligomerization of Sticholysins from Förster Resonance Energy Transfer.Biochemistry · 2021Article
- Role of Lipids in Morphogenesis of T-Cell Microvilli.Frontiers in immunology · 2021Review
- Functional and Structural Variation among Sticholysins, Pore-Forming Proteins from the Sea AnemoneInternational journal of molecular sciences · 2020Review
- Cholesterol-Induced Conformational Change in the Sphingomyelin Headgroup.Biophysical journal · 2019Article
- Lateral Segregation of Palmitoyl Ceramide-1-Phosphate in Simple and Complex Bilayers.Biophysical journal · 2019Article
- Alteration of Membrane Physicochemical Properties by Two Factors for Membrane Protein Integration.Biophysical journal · 2019Article
- Pore-Forming Proteins from Cnidarians and Arachnids as Potential Biotechnological Tools.Toxins · 2019Article
- Sticholysin, Sphingomyelin, and Cholesterol: A Closer Look at a Tripartite Interaction.Biophysical journal · 2019Article
- Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.Chemical reviews · 2019Article
- Natural Ceramides and Lysophospholipids Cosegregate in Fluid Phosphatidylcholine Bilayers.Biophysical journal · 2019Article
- Characterization of Plasma Membrane Ceramides by Super-Resolution Microscopy.Angewandte Chemie (International ed. in English) · 2017Article
- Bilayer Interactions among Unsaturated Phospholipids, Sterols, and Ceramide.Biophysical journal · 2017Article
- Detection of Sphingomyelin Clusters by Raman Spectroscopy.Biophysical journal · 2016Article
- The Functional Activity of the Human Serotonin 5-HT1A Receptor Is Controlled by Lipid Bilayer Composition.Biophysical journal · 2016Article
- Lipid Interactions and Organization in Complex Bilayer Membranes.Biophysical journal · 2016Article
- The Influence of Hydrogen Bonding on Sphingomyelin/Colipid Interactions in Bilayer Membranes.Biophysical journal · 2016Article
- There Is No Simple Model of the Plasma Membrane Organization.Frontiers in cell and developmental biology · 2016Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sphingomyelins (SMs) and sterols are important constituents of the plasma membrane and have also been identified as major lipid components in membrane rafts. Using SM analogs with decreasing headgroup methylation, we systemically analyzed the effect of headgroup size on membrane properties and interactions with cholesterol. An increase in headgroup size resulted in a decrease in the main phase transition. Atom-scale molecular-dynamics simulations were in agreement with the fluorescence anisotropy experiments, showing that molecular areas increased and acyl chain order decreased with increasing headgroup size. Furthermore, the transition temperatures were constantly higher for SM headgroup analogs compared to corresponding phosphatidylcholine headgroup analogs. The sterol affinity for phospholipid bilayers was assessed using a sterol-partitioning assay and an increased headgroup size increased sterol affinity for the bilayer, with a higher sterol affinity for SM analogs as compared to phosphatidylcholine analogs. Moreover, the size of the headgroup affected the formation and composition of cholesterol-containing ordered domains. Palmitoyl-SM (the largest headgroup) seemed to attract more cholesterol into ordered domains than the other SM analogs with smaller headgroups. The ordering and condensing effect of cholesterol on membrane lipids was also largest for palmitoyl-SM as compared to the smaller SM analogs. The results show that the size of the SM headgroup is crucially important for SM-SM and SM-sterol interactions. Our results further emphasize that interfacial electrostatic interactions are important for stabilizing cholesterol interactions with SMs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.