Evidence map›Paper›PMID 21080963›Full record

ArticleBMC biochemistry2010

A sequence-dependent exonuclease activity from Tetrahymena thermophila.

Hui-I Kao Tom, Carol W Greider

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Hui-I Kao TomDepartment of Molecular Biology and Genetics, The Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA.
Carol W Greider
Johns Hopkins Medicine · USJohns Hopkins University · US

Funding

STRUCTURE/FUNCTION OF TELOMERES IN MAMMALIAN AGINGR37AG009383 · NIA · JOHNS HOPKINS UNIVERSITY · PI GREIDER, CAROL W · 1997 to 2016
$6.1M
STRUCTURE AND FUNCTION OF TELOMERES IN MAMMALIAN AGINGR01AG009383 · NIA · COLD SPRING HARBOR LABORATORY · PI GREIDER, CAROL W · 1991 to 1996
–
NIA NIH HHS R01AG09383NIA NIH HHS R37 AG009383
6 · The paper itself

Abstract

backgroundTelomere function requires a highly conserved G rich 3'- overhang. This structure is formed by 5'-resection of the C-rich telomere strand. However, while many nucleases have been suggested to play a role in processing, it is not yet clear which nucleases carry out this 5'-resection.

resultsWe used biochemical purification to identify a sequence-dependent exonuclease activity in Tetrahymena thermophila cell extracts. The nuclease activity showed specificity for 5'-ends containing AA or AC sequences, unlike Exo1, which showed sequence-independent cleavage. The Tetrahymena nuclease was active on both phosphorylated and unphosphorylated substrates whereas Exo1 requires a 5'-phosphate for cleavage.

conclusionsThe specificities of the enzyme indicate that this novel Tetrahymena exonuclease is distinct from Exo1 and has properties required for 3'-overhang formations at telomeres.

Indexed as

Base SequenceDNAExodeoxyribonucleasesExonucleasesGC Rich SequenceNucleotidesSubstrate SpecificityTelomereTetrahymena thermophilaDNAExodeoxyribonucleasesExonucleasesNucleotides

Identifiers

PMID21080963
PMCPMC2998447
OpenAlexW2150430826

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.