ArticleBMC microbiology2010
A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- IL-6 Degradation by Secreted Proteases FromInternational journal of microbiology · 2025Article
- Iron Deprivation Modulates the Exoproteome inFrontiers in cellular and infection microbiology · 2022Article
- Updates inJournal of fungi (Basel, Switzerland) · 2021Review
- Gene expression of Paracoccidioides virulence factors after interaction with macrophages and fibroblasts.Memorias do Instituto Oswaldo Cruz · 2021Article
- The Trojan Horse Model inFrontiers in cellular and infection microbiology · 2020Review
- Proteomic Analysis ofFrontiers in microbiology · 2019Article
- Immunoproteomic Approach of Extracellular Antigens FromFrontiers in microbiology · 2019Article
- Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection.Virulence · 2017Article
- Fungal lifestyle reflected in serine protease repertoire.Scientific reports · 2017Article
- Fungi that Infect Humans.Microbiology spectrum · 2017Review
- Paracoccidioides brasiliensis induces cytokine secretion in epithelial cells in a protease-activated receptor-dependent (PAR) manner.Medical microbiology and immunology · 2017Article
- Article
- In vitro Paracoccidioides brasiliensis biofilm and gene expression of adhesins and hydrolytic enzymes.Virulence · 2015Article
- Predicting copper-, iron-, and zinc-binding proteins in pathogenic species of the Paracoccidioides genus.Frontiers in microbiology · 2014Article
- Identification of human plasma proteins associated with the cell wall of the pathogenic fungus Paracoccidioides brasiliensis.FEMS microbiology letters · 2013Article
- Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundParacoccidioides brasiliensis is a thermodimorphic fungus, the causative agent of paracoccidioidomycosis (PCM). Serine proteases are widely distributed and this class of peptidase has been related to pathogenesis and nitrogen starvation in pathogenic fungi.
resultsA cDNA (Pbsp) encoding a secreted serine protease (PbSP), was isolated from a cDNA library constructed with RNAs of fungal yeast cells recovered from liver of infected mice. Recombinant PbSP was produced in Escherichia coli, and used to develop polyclonal antibodies that were able to detect a 66 kDa protein in the P. brasiliensis proteome. In vitro deglycosylation assays with endoglycosidase H demonstrated that PbSP is a N-glycosylated molecule. The Pbsp transcript and the protein were induced during nitrogen starvation. The Pbsp transcript was also induced in yeast cells infecting murine macrophages. Interactions of PbSP with P. brasiliensis proteins were evaluated by two-hybrid assay in the yeast Saccharomyces cerevisiae. PbSP interacts with a peptidyl prolyl cis-trans isomerase, calnexin, HSP70 and a cell wall protein PWP2.
conclusionsA secreted subtilisin induced during nitrogen starvation was characterized indicating the possible role of this protein in the nitrogen acquisition. PbSP interactions with other P. brasiliensis proteins were reported. Proteins interacting with PbSP are related to folding process, protein trafficking and cytoskeleton reorganization.
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