ArticlePLoS genetics2010
Genetic basis of growth adaptation of Escherichia coli after deletion of pgi, a major metabolic gene.
Article in PLoS genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
78 citing papers in PubMed.
- Metabolite correlation networks reveal complex phenotypes of adaptation-driving mutations.Nature communications · 2026Article
- Diversity of Transcriptional Regulatory Adaptation in E. coli.Molecular biology and evolution · 2024Article
- RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024Review
- A citric acid cycle-deficient Escherichia coli as an efficient chassis for aerobic fermentations.Nature communications · 2024Article
- Autotrophic growth ofeLife · 2024Article
- Investigating the mechanism of rough phenotype in a naturally attenuatedFrontiers in medicine · 2024Article
- Physiological and transcriptional studies reveal Cr(VI) reduction mechanisms in the exoelectrogenFrontiers in microbiology · 2023Article
- Characterization of an Entner-Doudoroff pathway-activated Escherichia coli.Biotechnology for biofuels and bioproducts · 2022Article
- Rapid Adaptation Often Occurs through Mutations to the Most Highly Conserved Positions of the RNA Polymerase Core Enzyme.Genome biology and evolution · 2022Article
- A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii.Communications biology · 2022Article
- Global pleiotropic effects in adaptively evolvedOpen biology · 2022Article
- Enhancing protein perdeuteration by experimental evolution of Escherichia coli K-12 for rapid growth in deuterium-based media.Protein science : a publication of the Protein Society · 2021Article
- Experimental evolution of extremophile resistance to ionizing radiation.Trends in genetics : TIG · 2021Review
- Setting the stage for evolution of a new enzyme.Current opinion in structural biology · 2021Review
- Starvation induces shrinkage of the bacterial cytoplasm.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Dynamic control in metabolic engineering: Theories, tools, and applications.Metabolic engineering · 2021Review
- Deletion of pgi gene in E. coli increases tolerance to furfural and 5-hydroxymethyl furfural in media containing glucose-xylose mixture.Microbial cell factories · 2020Article
- Causal mutations from adaptive laboratory evolution are outlined by multiple scales of genome annotations and condition-specificity.BMC genomics · 2020Article
- Metabolic role of pyrophosphate-linked phosphofructokinase pfk for C1 assimilation in Methylotuvimicrobium alcaliphilum 20Z.Microbial cell factories · 2020Article
- Evolutionary Repair Experiments as a Window to the Molecular Diversity of Life.Current biology : CB · 2020Review
18 more citing papers are in PubMed but not listed here.
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8 authors.
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Abstract
Bacterial survival requires adaptation to different environmental perturbations such as exposure to antibiotics, changes in temperature or oxygen levels, DNA damage, and alternative nutrient sources. During adaptation, bacteria often develop beneficial mutations that confer increased fitness in the new environment. Adaptation to the loss of a major non-essential gene product that cripples growth, however, has not been studied at the whole-genome level. We investigated the ability of Escherichia coli K-12 MG1655 to overcome the loss of phosphoglucose isomerase (pgi) by adaptively evolving ten replicates of E. coli lacking pgi for 50 days in glucose M9 minimal medium and by characterizing endpoint clones through whole-genome re-sequencing and phenotype profiling. We found that 1) the growth rates for all ten endpoint clones increased approximately 3-fold over the 50-day period; 2) two to five mutations arose during adaptation, most frequently in the NADH/NADPH transhydrogenases udhA and pntAB and in the stress-associated sigma factor rpoS; and 3) despite similar growth rates, at least three distinct endpoint phenotypes developed as defined by different rates of acetate and formate secretion. These results demonstrate that E. coli can adapt to the loss of a major metabolic gene product with only a handful of mutations and that adaptation can result in multiple, alternative phenotypes.
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