ArticleThe Journal of general physiology2010
Unitary Ca(2+) current through recombinant type 3 InsP(3) receptor channels under physiological ionic conditions.
Article in The Journal of general physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Stochastic model of IP3-induced Ca2+ spiking of HEK293 cells.PLoS computational biology · 2025Article
- Modeling traveling calcium waves in cellular structures.Journal of computational neuroscience · 2025Article
- Quantal CaCell reports · 2021Article
- ER-luminal [CaeLife · 2020Article
- Intracellular calcium stores mediate metaplasticity at hippocampal dendritic spines.The Journal of physiology · 2019Article
- Structure and Function of IPCold Spring Harbor perspectives in biology · 2019Review
- All three IPJournal of cell science · 2018Article
- MICU2 Restricts Spatial Crosstalk between InsPCell reports · 2017Article
- Spatial CaThe Journal of physiology · 2017Review
- Mode switching of Inositol 1,4,5-trisphosphate receptor channel shapes the Spatiotemporal scales of CaJournal of biological physics · 2016Article
- Characterization of Two-Pore Channel 2 by Nuclear Membrane Electrophysiology.Scientific reports · 2016Article
- Inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum: A single-channel point of view.Cell calcium · 2015Review
- Optimal microdomain crosstalk between endoplasmic reticulum and mitochondria for Ca2+ oscillations.Scientific reports · 2015Article
- Modulation of elementary calcium release mediates a transition from puffs to waves in an IP3R cluster model.PLoS computational biology · 2015Article
- Nuclear patch-clamp electrophysiology of Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- Patch-clamp electrophysiology of intracellular Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- Quercetin as a fluorescent probe for the ryanodine receptor activity in Jurkat cells.Pflugers Archiv : European journal of physiology · 2013Article
- Lysosomes shape Ins(1,4,5)P3-evoked Ca2+ signals by selectively sequestering Ca2+ released from the endoplasmic reticulum.Journal of cell science · 2013Article
- Permeant calcium ion feed-through regulation of single inositol 1,4,5-trisphosphate receptor channel gating.The Journal of general physiology · 2012Article
- Multi-scale data-driven modeling and observation of calcium puffs.Cell calcium · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ubiquitous inositol 1,4,5-trisphosphate (InsP(3)) receptor (InsP(3)R) channel, localized primarily in the endoplasmic reticulum (ER) membrane, releases Ca(2+) into the cytoplasm upon binding InsP(3), generating and modulating intracellular Ca(2+) signals that regulate numerous physiological processes. Together with the number of channels activated and the open probability of the active channels, the size of the unitary Ca(2+) current (i(Ca)) passing through an open InsP(3)R channel determines the amount of Ca(2+) released from the ER store, and thus the amplitude and the spatial and temporal nature of Ca(2+) signals generated in response to extracellular stimuli. Despite its significance, i(Ca) for InsP(3)R channels in physiological ionic conditions has not been directly measured. Here, we report the first measurement of i(Ca) through an InsP(3)R channel in its native membrane environment under physiological ionic conditions. Nuclear patch clamp electrophysiology with rapid perfusion solution exchanges was used to study the conductance properties of recombinant homotetrameric rat type 3 InsP(3)R channels. Within physiological ranges of free Ca(2+) concentrations in the ER lumen ([Ca(2+)](ER)), free cytoplasmic [Ca(2+)] ([Ca(2+)](i)), and symmetric free [Mg(2+)] ([Mg(2+)](f)), the i(Ca)-[Ca(2+)](ER) relation was linear, with no detectable dependence on [Mg(2+)](f). i(Ca) was 0.15 +/- 0.01 pA for a filled ER store with 500 microM [Ca(2+)](ER). The i(Ca)-[Ca(2+)](ER) relation suggests that Ca(2+) released by an InsP(3)R channel raises [Ca(2+)](i) near the open channel to approximately 13-70 microM, depending on [Ca(2+)](ER). These measurements have implications for the activities of nearby InsP(3)-liganded InsP(3)R channels, and they confirm that Ca(2+) released by an open InsP(3)R channel is sufficient to activate neighboring channels at appropriate distances away, promoting Ca(2+)-induced Ca(2+) release.
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