ArticleGenetics2011
Transgene expression and transgene-induced silencing in diploid and autotetraploid Arabidopsis.
Article in Genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Asymmetric bulges within hairpin RNA transgenes influence small RNA size, secondary siRNA production and viral defence.Nucleic acids research · 2024Article
- Minimizing IP issues associated with gene constructs encoding the Bt toxin - a case study.BMC biotechnology · 2024Article
- Nucleotide mismatches prevent intrinsic self-silencing of hpRNA transgenes to enhance RNAi stability in plants.Nature communications · 2022Article
- Functional Characterization of Genes Coding for NovelFrontiers in plant science · 2022Article
- An Arabidopsis AT-hook motif nuclear protein mediates somatic embryogenesis and coinciding genome duplication.Nature communications · 2021Article
- Comprehensive Mechanism of Gene Silencing and Its Role in Plant Growth and Development.Frontiers in plant science · 2021Review
- Article
- Expression patterns ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Cloning, transformation and expression of cell cycle-associated protein kinaseJournal, genetic engineering & biotechnology · 2018Article
- Article
- Phosphatidylinositol 3-Kinase-DNA Methyltransferase 1-miR-1281-Histone Deacetylase 4 Regulatory Axis Mediates Platelet-Derived Growth Factor-Induced Proliferation and Migration of Pulmonary Artery Smooth Muscle Cells.Journal of the American Heart Association · 2018Article
- DNA-Demethylase Regulated Genes Show Methylation-Independent Spatiotemporal Expression Patterns.Frontiers in plant science · 2017Article
- CaMV-35S promoter sequence-specific DNA methylation in lettuce.Plant cell reports · 2016Article
- Nicotiana small RNA sequences support a host genome origin of cucumber mosaic virus satellite RNA.PLoS genetics · 2015Article
- Epigenetic silencing in transgenic plants.Frontiers in plant science · 2015Review
- DNA demethylases target promoter transposable elements to positively regulate stress responsive genes in Arabidopsis.Genome biology · 2014Article
- Ploidy and Hybridity Effects on Growth Vigor and Gene Expression in Arabidopsis thaliana Hybrids and Their Parents.G3 (Bethesda, Md.) · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have suggested that transgene expression in plants can be affected by ploidy. Here we show that three different transgenes, a reporter transgene, an antisense transgene, and a hairpin RNA (hpRNA) transgene, are all expressed at a lower level in autotetraploid (4n) than in diploid (2n) Arabidopsis. RNA silencing of two endogenous genes was induced by the antisense and hpRNA transgenes and this silencing is significantly less effective in 4n than in 2n Arabidopsis; furthermore, the reduced silencing in 4n Arabidopsis correlated with reduced accumulation of silencing-inducer RNAs. Methylation analysis both of independent 2n and 4n transgenic lines and of 2n and 4n progeny derived from the same 3n transgenic parent, indicated that transgenes are more methylated in 4n than 2n Arabidopsis. These results suggest that transgenes are transcriptionally repressed in the 4n background, resulting in expression levels lower than in the 2n background. Transgenes designed to silence endogenous genes express lower concentrations of silencing-inducer RNAs in 4n Arabidopsis plants, resulting in less effective silencing of target genes than in 2n Arabidopsis plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.