ArticleThe Journal of biological chemistry2011
MyoD directly up-regulates premyogenic mesoderm factors during induction of skeletal myogenesis in stem cells.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
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- Chemical genetics reveals cross-activation of plant developmental signaling by the immune peptide-receptor pathway.bioRxiv : the preprint server for biology · 2024Article
- Possible involvement of zinc transporter ZIP13 in myogenic differentiation.Scientific reports · 2024Article
- Combination of cell signaling molecules can facilitate MYOD1-mediated myogenic transdifferentiation of pig fibroblasts.Journal of animal science and biotechnology · 2021Article
- Mutational burden and potential oligogenic model of TBX6-mediated genes in congenital scoliosis.Molecular genetics & genomic medicine · 2020Article
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- Induction of Skeletal Muscle Progenitors and Stem Cells from human induced Pluripotent Stem Cells.Journal of neuromuscular diseases · 2020Review
- Skeletal Muscle Pathophysiology: The Emerging Role of Spermine Oxidase and Spermidine.Medical sciences (Basel, Switzerland) · 2018Review
- Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.Scientific reports · 2018Article
- Skeletal Muscle Cell Induction from Pluripotent Stem Cells.Stem cells international · 2017Review
- BRG1 interacts with GLI2 and binds Mef2c gene in a hedgehog signalling dependent manner during in vitro cardiomyogenesis.BMC developmental biology · 2016Article
- Hedgehog/Patched-associated rhabdomyosarcoma formation from delta1-expressing mesodermal cells.Oncogene · 2016Article
- Myogenic Differentiation of Mouse Embryonic Stem Cells That Lack a Functional Pax7 Gene.Stem cells and development · 2016Article
- Time course and side-by-side analysis of mesodermal, pre-myogenic, myogenic and differentiated cell markers in the chicken model for skeletal muscle formation.Journal of anatomy · 2015Article
- The emergence of Pax7-expressing muscle stem cells during vertebrate head muscle development.Frontiers in aging neuroscience · 2015Article
- AP-1-mediated expression of brain-specific class IVa β-tubulin in P19 embryonal carcinoma cells.The Journal of veterinary medical science · 2014Article
- Transcriptome of Atoh7 retinal progenitor cells identifies new Atoh7-dependent regulatory genes for retinal ganglion cell formation.Developmental neurobiology · 2014Article
- Developmental transcriptome analysis of human erythropoiesis.Human molecular genetics · 2014Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Gain- and loss-of-function experiments have illustrated that the family of myogenic regulatory factors is necessary and sufficient for the formation of skeletal muscle. Furthermore, MyoD required cellular aggregation to induce myogenesis in P19 embryonal carcinoma stem cells. To determine the mechanism by which stem cells can be directed into skeletal muscle, a time course of P19 cell differentiation was examined in the presence and absence of exogenous MyoD. By quantitative PCR, the first MyoD up-regulated transcripts were the premyogenic mesoderm factors Meox1, Pax7, Six1, and Eya2 on day 4 of differentiation. Subsequently, the myoblast markers myogenin, MEF2C, and Myf5 were up-regulated, leading to skeletal myogenesis. These results were corroborated by Western blot analysis, showing up-regulation of Pax3, Six1, and MEF2C proteins, prior to myogenin protein expression. To determine at what stage a dominant-negative MyoD/EnR mutant could inhibit myogenesis, stable cell lines were created and examined. Interestingly, the premyogenic mesoderm factors, Meox1, Pax3/7, Six1, Eya2, and Foxc1, were down-regulated, and as expected, skeletal myogenesis was abolished. Finally, to identify direct targets of MyoD in this system, chromatin immunoprecipitation experiments were performed. MyoD was observed associated with regulatory regions of Meox1, Pax3/7, Six1, Eya2, and myogenin genes. Taken together, MyoD directs stem cells into the skeletal muscle lineage by binding and activating the expression of premyogenic mesoderm genes, prior to activating myoblast genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.