ArticleThe Journal of biological chemistry2011
Histone deacetylase 9 activates gamma-globin gene expression in primary erythroid cells.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Histone modifications in the regulation of erythropoiesis.Annals of medicine · 2025Review
- Comprehensive regulation of γ-globin expression by epigenetic modifications and protein post-translational modifications.Clinical epigenetics · 2025Review
- Butyrate Prevents Obesity Accompanied by HDAC9-Mediated Browning of White Adipose Tissue.Biomedicines · 2025Article
- Pharmacogenomics of Drugs Used in β-Thalassemia and Sickle-Cell Disease: From Basic Research to Clinical Applications.International journal of molecular sciences · 2024Review
- Histone Deacetylases Function in the Control of Early Hematopoiesis and Erythropoiesis.International journal of molecular sciences · 2022Review
- Interplay between cofactors and transcription factors in hematopoiesis and hematological malignancies.Signal transduction and targeted therapy · 2021Review
- Role of HDACs in normal and malignant hematopoiesis.Molecular cancer · 2020Review
- Omics Studies in Hemoglobinopathies.Molecular diagnosis & therapy · 2019Review
- Megakaryocyte lineage development is controlled by modulation of protein acetylation.PloS one · 2018Article
- Original Research: A case-control genome-wide association study identifies genetic modifiers of fetal hemoglobin in sickle cell disease.Experimental biology and medicine (Maywood, N.J.) · 2016Article
- HDAC7 modulates TNF-α-mediated suppression of Leydig cell steroidogenesis.Molecular and cellular biochemistry · 2015Article
- A cell-based high-throughput screen for novel chemical inducers of fetal hemoglobin for treatment of hemoglobinopathies.PloS one · 2014Article
- A network of epigenetic regulators guides developmental haematopoiesis in vivo.Nature cell biology · 2013Article
- Transcriptional environment and chromatin architecture interplay dictates globin expression patterns of heterospecific hybrids derived from undifferentiated human embryonic stem cells or from their erythroid progeny.Experimental hematology · 2013Article
- Regulation of Gγ-globin gene by ATF2 and its associated proteins through the cAMP-response element.PloS one · 2013Article
- Histone deacetylases 2 and 9 are coexpressed and nuclear localized in human molar odontoblasts in vivo.Histochemistry and cell biology · 2012Article
- Specific control of pancreatic endocrine β- and δ-cell mass by class IIa histone deacetylases HDAC4, HDAC5, and HDAC9.Diabetes · 2011Article
- Histone deacetylase 9 is a negative regulator of adipogenic differentiation.The Journal of biological chemistry · 2011Article
- Krüppel-like Factor 4 activates HBG gene expression in primary erythroid cells.British journal of haematology · 2011Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Strategies to induce fetal hemoglobin (HbF) synthesis for the treatment of β-hemoglobinopathies probably involve protein modifications by histone deacetylases (HDACs) that mediate γ-globin gene regulation. However, the role of individual HDACs in globin gene expression is not very well understood; thus, the focus of our study was to identify HDACs involved in γ-globin activation. K562 erythroleukemia cells treated with the HbF inducers hemin, trichostatin A, and sodium butyrate had significantly reduced mRNA levels of HDAC9 and its splice variant histone deacetylase-related protein. Subsequently, HDAC9 gene knockdown produced dose-dependent γ-globin gene silencing over an 80-320 nm range. Enforced expression with the pTarget-HDAC9 vector produced a dose-dependent 2.5-fold increase in γ-globin mRNA (p < 0.05). Furthermore, ChIP assays showed HDAC9 binding in vivo in the upstream Gγ-globin gene promoter region. To determine the physiological relevance of these findings, human primary erythroid progenitors were treated with HDAC9 siRNA; we observed 40 and 60% γ-globin gene silencing in day 11 (early) and day 28 (late) progenitors. Moreover, enforced HDAC9 expression increased γ-globin mRNA levels by 2.5-fold with a simultaneous 7-fold increase in HbF. Collectively, these data support a positive role for HDAC9 in γ-globin gene regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.