ArticleAnalytical biochemistry2011
Applications of phasors to in vitro time-resolved fluorescence measurements.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 87 citations in OpenAlex.
- Phasor theory of fluorescence lifetime imaging utilized on a maximum range of frequencies for prostate tissue analysis.Biomedical optics express · 2025Article
- Exploring the Binding Mechanism of 5-HT7 Specific Benzoxazolone alkyl Piperazinium Derivatives: A Comprehensive Analysis Using Spectroscopic and Computational Approaches.Journal of fluorescence · 2025Article
- Fluorescence phasor analysis: basic principles and biophysical applications.Biophysical reviews · 2025Review
- PMP22 duplication dysregulates lipid homeostasis and plasma membrane organization in developing human Schwann cells.Brain : a journal of neurology · 2024Article
- Blind instrument response function identification from fluorescence decays.Biophysical reports · 2024Review
- Formalin fixation and paraffin embedding interfere with the preservation of optical metabolic assessments based on endogenous NAD(P)H and FAD two-photon excited fluorescence.Biomedical optics express · 2023Article
- Biomolecular condensates modulate membrane lipid packing and hydration.Nature communications · 2023Article
- Critical Beginnings: Selective Tuning of Solubility and Structural Accuracy of Newly Synthesized Proteins by the Hsp70 Chaperone System.The journal of physical chemistry. B · 2023Article
- Fluorescence lifetime: Beating the IRF and interpulse window.Biophysical journal · 2023Article
- Fluorescence Lifetime Phasor Analysis of the Decamer-Dimer Equilibrium of Human Peroxiredoxin 1.International journal of molecular sciences · 2022Article
- An EfficientInternational journal of molecular sciences · 2022Article
- Development and characterization of phasor-based analysis for FLIM to evaluate the metabolic and epigenetic impact of HER2 inhibition on squamous cell carcinoma cultures.Journal of biomedical optics · 2021Article
- AKAP13 couples GPCR signaling to mTORC1 inhibition.PLoS genetics · 2021Article
- Quantitation of cerebral oxygen tension using phasor analysis and phosphorescence lifetime imaging microscopy (PLIM).Biomedical optics express · 2021Article
- Direct Photon-by-Photon Analysis of Time-Resolved Pulsed Excitation Data using Bayesian Nonparametrics.Cell reports. Physical science · 2020Article
- Blind Resolution of Lifetime Components in Individual Pixels of Fluorescence Lifetime Images Using the Phasor Approach.The journal of physical chemistry. B · 2020Article
- Full-Harmonics Phasor Analysis: Unravelling Multiexponential Trends in Magnetic Resonance Imaging Data.The journal of physical chemistry letters · 2020Article
- Spatial and temporal resolution of mORC4 fluorescent variants reveals structural requirements for achieving higher order self-association and pronuclei entry.Methods and applications in fluorescence · 2019Article
- Evaluating Cell Metabolism Through Autofluorescence Imaging of NAD(P)H and FAD.Antioxidants & redox signaling · 2019Review
- Gramicidin Lateral Distribution in Phospholipid Membranes: Fluorescence Phasor Plots and Statistical Mechanical Model.International journal of molecular sciences · 2018Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The phasor method of treating fluorescence lifetime data provides a facile and convenient approach to characterize lifetime heterogeneity and to detect the presence of excited state reactions such as solvent relaxation and Förster resonance energy transfer. The method uses a plot of M sin(Φ) versus M cos(Φ), where M is the modulation ratio and Φ is the phase angle taken from frequency domain fluorometry. A principal advantage of the phasor method is that it provides a model-less approach to time-resolved data amenable to visual inspection. Although the phasor approach has been recently applied to fluorescence lifetime imaging microscopy, it has not been used extensively for cuvette studies. In the current study, we explore the applications of the method to in vitro samples. The phasors of binary and ternary mixtures of fluorescent dyes demonstrate the utility of the method for investigating complex mixtures. Data from excited state reactions, such as dipolar relaxation in membrane and protein systems and also energy transfer from the tryptophan residue to the chromophore in enhanced green fluorescent protein, are also presented.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.