ArticleAnalytical biochemistry2011
Applications of phasor plots to in vitro protein studies.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Studying biological events using biopolymeric matrices.Biophysical reviews · 2025Review
- Fluorescence phasor analysis: basic principles and biophysical applications.Biophysical reviews · 2025Review
- Biomolecular condensates modulate membrane lipid packing and hydration.Nature communications · 2023Article
- Critical Beginnings: Selective Tuning of Solubility and Structural Accuracy of Newly Synthesized Proteins by the Hsp70 Chaperone System.The journal of physical chemistry. B · 2023Article
- Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Fluorescence Lifetime Phasor Analysis of the Decamer-Dimer Equilibrium of Human Peroxiredoxin 1.International journal of molecular sciences · 2022Article
- LAURDAN since Weber: The Quest for Visualizing Membrane Heterogeneity.Accounts of chemical research · 2021Article
- Wide-field time-gated SPAD imager for phasor-based FLIM applications.Methods and applications in fluorescence · 2020Article
- Spatial and temporal resolution of mORC4 fluorescent variants reveals structural requirements for achieving higher order self-association and pronuclei entry.Methods and applications in fluorescence · 2019Article
- In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short-lifetime near-infrared dyes and time-gated imaging.Journal of biophotonics · 2019Article
- Gramicidin Lateral Distribution in Phospholipid Membranes: Fluorescence Phasor Plots and Statistical Mechanical Model.International journal of molecular sciences · 2018Article
- Characterization of esterase activity from an Acetomicrobium hydrogeniformans enzyme with high structural stability in extreme conditions.Extremophiles : life under extreme conditions · 2018Article
- Differences between FLIM phasor analyses for data collected with the Becker and Hickl SPC830 card and with the FLIMbox card.Microscopy research and technique · 2018Article
- Aggregation-primed molten globule conformers of the p53 core domain provide potential tools for studying p53C aggregation in cancer.The Journal of biological chemistry · 2018Article
- A multidimensional phasor approach reveals LAURDAN photophysics in NIH-3T3 cell membranes.Scientific reports · 2017Article
- Measuring the effect of a Western diet on liver tissue architecture by FLIM autofluorescence and harmonic generation microscopy.Biomedical optics express · 2017Article
- Environmental Factors Modulating the Stability and Enzymatic Activity of the Petrotoga mobilis Esterase (PmEst).PloS one · 2016Article
- Model-free methods to study membrane environmental probes: a comparison of the spectral phasor and generalized polarization approaches.Methods and applications in fluorescence · 2015Article
- Axial super resolution topography of focal adhesion by confocal microscopy.Microscopy research and technique · 2013Article
- The effect of translational motion on FLIM measurements-single particle phasor-FLIM.Journal of fluorescence · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In a recent article, we described the application of phasor analysis to fluorescence intensity decay data on in vitro samples. As detailed in that article, this method provides researchers with a simple graphical method for viewing lifetime data that can be used to quantify individual components of a mixture as well as to identify excited state reactions. In the current article, we extend the use of in vitro phasor analysis to intrinsic protein fluorescence. We show how alterations in the excited state properties of tryptophan residues are easily visualized using the phasor method. Specifically, we demonstrate that protein-ligand and protein-protein interactions can result in unique shifts in the location of phasor points, indicative of protein conformational changes. Application of the method to a rapid kinetic experiment is also shown. Finally, we show that the unfolding of lysozyme with either urea or guanidine hydrochloride results in different phasor trajectories, indicative of unique denaturation pathways.
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