ArticleAnalytical biochemistry2011
Optimization of antibody-conjugated magnetic nanoparticles for target preconcentration and immunoassays.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 57 citations in OpenAlex.
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- Exploiting the Potential of Magnetic Nanoparticles for Rapid Diagnosis Tests (RDTs): Nanoparticle-Antibody Conjugates and Color Development Strategies.Diagnostics (Basel, Switzerland) · 2023Article
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- Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process.Frontiers in bioengineering and biotechnology · 2022Review
- Engineering magnetic nanoparticles and their integration with microfluidics for cell isolation.Journal of colloid and interface science · 2020Article
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- A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies.Nanoscale research letters · 2017Article
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- High performance wash-free magnetic bioassays through microfluidically enhanced particle specificity.Scientific reports · 2015Article
- Nano-magnetic immunosensor based on staphylococcus protein a and the amplification effect of HRP-conjugated phage antibody.Sensors (Basel, Switzerland) · 2015Article
- Rapid immunoenzyme assay of aflatoxin B1 using magnetic nanoparticles.Sensors (Basel, Switzerland) · 2014Article
- Miniaturized protein microarray with internal calibration as point-of-care device for diagnosis of neonatal sepsis.Sensors (Basel, Switzerland) · 2012Article
- Transforming the blood glucose meter into a general healthcare meter for in vitro diagnostics in mobile health.Biotechnology advancesReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
Biosensors based on antibody recognition have a wide range of monitoring applications that apply to clinical, environmental, homeland security, and food problems. In an effort to improve the limit of detection of the Naval Research Laboratory (NRL) Array Biosensor, magnetic nanoparticles (MNPs) were designed and tested using a fluorescence-based array biosensor. The MNPs were coated with the fluorescently labeled protein, AlexaFluor647-chicken IgG (Alexa647-chick IgG). Antibody-labeled MNPs (Alexa647-chick-MNPs) were used to preconcentrate the target via magnetic separation and as the tracer to demonstrate binding to slides modified with anti-chicken IgG as a capture agent. A full optimization study of the antibody-modified MNPs and their use in the biosensor was performed. This investigation looked at the Alexa647-chick-MNP composition, MNP surface modifications, target preconcentration conditions, and the effect that magnetic extraction has on the Alexa647-chick-MNP binding with the array surface. The results demonstrate the impact of magnetic extraction using the MNPs labeled with fluorescent proteins both for target preconcentration and for subsequent integration into immunoassays performed under flow conditions for enhanced signal generation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.