ArticleBiochemistry2010
Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Hydrogen Bonding and Noncovalent Electric Field Effects in the Photoconversion of a Phytochrome.The journal of physical chemistry. B · 2024Article
- Copper-catalyzed asymmetric C(spNature communications · 2023Article
- Site-Specific Interrogation of Protein Structure and Stability.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cyanylated Cysteine Reports Site-Specific Changes at Protein-Protein-Binding Interfaces Without Perturbation.Biochemistry · 2018Article
- Visible-Light-Mediated Selective Arylation of Cysteine in Batch and Flow.Angewandte Chemie (International ed. in English) · 2017Article
- Simple method to introduce an ester infrared probe into proteins.Protein science : a publication of the Protein Society · 2017Article
- Minimalist IR and fluorescence probes of protein function.Current opinion in chemical biology · 2016Review
- Two-Dimensional Infrared Study of Vibrational Coupling between Azide and Nitrile Reporters in a RNA Nucleoside.The journal of physical chemistry. B · 2016Article
- Site-specific infrared probes of proteins.Annual review of physical chemistry · 2015Review
- General strategy for the bioorthogonal incorporation of strongly absorbing, solvation-sensitive infrared probes into proteins.The journal of physical chemistry. B · 2014Article
- An alternative structural isoform in amyloid-like aggregates formed from thermally denatured human γD-crystallin.Protein science : a publication of the Protein Society · 2014Article
- A strongly absorbing class of non-natural labels for probing protein electrostatics and solvation with FTIR and 2D IR spectroscopies.The journal of physical chemistry. B · 2013Article
- Painting proteins blue: β-(1-azulenyl)-L-alanine as a probe for studying protein-protein interactions.Chemical communications (Cambridge, England) · 2013Article
- Site-specific measurement of water dynamics in the substrate pocket of ketosteroid isomerase using time-resolved vibrational spectroscopy.The journal of physical chemistry. B · 2012Article
- Vibrational stark effect of the electric-field reporter 4-mercaptobenzonitrile as a tool for investigating electrostatics at electrode/SAM/solution interfaces.International journal of molecular sciences · 2012Article
- Site-Specific Spectroscopic Reporters of the Local Electric Field, Hydration, Structure, and Dynamics of Biomolecules.The journal of physical chemistry letters · 2011Article
- A direct comparison of azide and nitrile vibrational probes.Physical chemistry chemical physics : PCCP · 2011Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alkylation or arylation reaction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.