ArticleSoil biology & biochemistry2010
Size Does Matter: Application-driven Approaches for Soil Metagenomics.
Article in Soil biology & biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Environmental DNA (eDNA) Technology in Biodiversity and Ecosystem Health Research: Advances and Prospects.Ecology and evolution · 2026Review
- The MetaGens algorithm for metagenomic database lossy compression and subject alignment.Database : the journal of biological databases and curation · 2023Article
- Designing Knowledge-Based Bioremediation Strategies Using Metagenomics.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next-Generation Sequencing Approach.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Functional characterization of prokaryotic dark matter: the road so far and what lies ahead.Current research in microbial sciences · 2022Review
- Metagenomic outlooks of microbial dynamics influenced by organic manure in tea garden soils of North Bengal, India.Archives of microbiology · 2021Article
- DominantFood science and biotechnology · 2021Article
- Article
- Novel 'Bacteriospray' Method Facilitates the Functional Screening of Metagenomic Libraries for Antimicrobial Activity.Methods and protocols · 2019Article
- Below-ground-above-ground Plant-microbial Interactions: Focusing on Soybean, Rhizobacteria and Mycorrhizal Fungi.The open microbiology journal · 2018Review
- Molecular perspectives and recent advances in microbial remediation of persistent organic pollutants.Environmental science and pollution research international · 2016Review
- Impact of long-term cropping of glyphosate-resistant transgenic soybean [Glycine max (L.) Merr.] on soil microbiome.Transgenic research · 2016Article
- An efficient and cost-effective method for DNA extraction from athalassohaline soil using a newly formulated cell extraction buffer.3 Biotech · 2016Article
- Dispersing misconceptions and identifying opportunities for the use of 'omics' in soil microbial ecology.Nature reviews. Microbiology · 2015Article
- Current and future resources for functional metagenomics.Frontiers in microbiology · 2015Article
- Targeted metagenomics as a tool to tap into marine natural product diversity for the discovery and production of drug candidates.Frontiers in microbiology · 2015Review
- Improved cultivation and metagenomics as new tools for bioprospecting in cold environments.Extremophiles : life under extreme conditions · 2015Review
- Biotic interactions in the rhizosphere: a diverse cooperative enterprise for plant productivity.Plant physiology · 2014Review
- Enhancing metagenomics investigations of microbial interactions with biofilm technology.International journal of molecular sciences · 2013Review
- Changes in diversity, abundance, and structure of soil bacterial communities in Brazilian Savanna under different land use systems.Microbial ecology · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Metagenomic analyses can provide extensive information on the structure, composition, and predicted gene functions of diverse environmental microbial assemblages. Each environment presents its own unique challenges to metagenomic investigation and requires a specifically designed approach to accommodate physicochemical and biotic factors unique to each environment that can pose technical hurdles and/or bias the metagenomic analyses. In particular, soils harbor an exceptional diversity of prokaryotes that are largely undescribed beyond the level of ribotype and are a potentially vast resource for natural product discovery. The successful application of a soil metagenomic approach depends on selecting the appropriate DNA extraction, purification, and if necessary, cloning methods for the intended downstream analyses. The most important technical considerations in a metagenomic study include obtaining a sufficient yield of high-purity DNA representing the targeted microorganisms within an environmental sample or enrichment and (if required) constructing a metagenomic library in a suitable vector and host. Size does matter in the context of the average insert size within a clone library or the sequence read length for a high-throughput sequencing approach. It is also imperative to select the appropriate metagenomic screening strategy to address the specific question(s) of interest, which should drive the selection of methods used in the earlier stages of a metagenomic project (e.g., DNA size, to clone or not to clone). Here, we present both the promising and problematic nature of soil metagenomics and discuss the factors that should be considered when selecting soil sampling, DNA extraction, purification, and cloning methods to implement based on the ultimate study objectives.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.