ArticleNature cell biology2010
Caenorhabditis elegans EFA-6 limits microtubule growth at the cell cortex.
Article in Nature cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 71 citations in OpenAlex.
- Divergence of Cortical Force-Generating Mechanisms Underlies Differences in Spindle Behavior betweenbioRxiv : the preprint server for biology · 2026Article
- Multiscale temporal tuning of force generation complex machinery governs cortical microtubule interactions during the first mitotic division inbioRxiv : the preprint server for biology · 2026Article
- The microtubule regulator EFA-6 forms cortical foci dependent on its intrinsically disordered region and interactions with tubulins.Cell reports · 2024Article
- KLP-7/Kinesin-13 orchestrates axon-dendrite checkpoints for polarized trafficking in neurons.Molecular biology of the cell · 2024Article
- The microtubule regulator EFA-6 forms spatially restricted cortical foci dependent on its intrinsically disordered region and interactions with tubulins.bioRxiv : the preprint server for biology · 2024Article
- Apical PAR protein caps orient the mitotic spindle in C. elegans early embryos.Current biology : CB · 2023Article
- Microtubules oppose cortical actomyosin-driven membrane ingression during C. elegans meiosis I polar body extrusion.PLoS genetics · 2023Article
- Cortical microtubules oppose actomyosin-driven membrane ingression duringbioRxiv : the preprint server for biology · 2023Article
- Apical PAR-3 caps orient the mitotic spindle inbioRxiv : the preprint server for biology · 2023Article
- Dynein localization and pronuclear movement in the C. elegans zygote.Cytoskeleton (Hoboken, N.J.) · 2022Article
- Cortical microtubule pulling forces contribute to the union of the parental genomes in theeLife · 2022Article
- A systematic analysis of microtubule-destabilizing factors during dendrite pruning in Drosophila.EMBO reports · 2021Article
- EFA6 in Axon Regeneration, as a Microtubule Regulator and as a Guanine Nucleotide Exchange Factor.Cells · 2021Review
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Microtubule Growth Rates Are Sensitive to Global and Local Changes in Microtubule Plus-End Density.Current biology : CB · 2020Article
- Polar relaxation by dynein-mediated removal of cortical myosin II.The Journal of cell biology · 2020Article
- Article
- Neuronal microtubules impact lifespan.Aging · 2019Article
- Microtubule regulators act in the nervous system to modulate fat metabolism and longevity through DAF-16 in C. elegans.Aging cell · 2019Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Microtubules are polymers of tubulin heterodimers that exhibit dynamic instability: periods of growth followed by periods of shrinkage. However, the molecular regulation of dynamic instability remains elusive. Here, we show that EFA-6, a cortically-localized protein, limits the growth of microtubules near the cell cortex of early embryonic cells from Caenorhabditis elegans, possibly by inducing microtubule catastrophes. Compared with wild type, embryos lacking EFA-6 had abnormally long and dense microtubules at the cell cortex, and growing microtubule plus ends resided at the cortex for up to five-fold longer. Loss of EFA-6 also caused excess centrosome separation and displacement towards the cell cortex early in mitosis, and subsequently a loss of anaphase spindle-pole oscillations and increased rates of spindle elongation. The centrosome separation phenotype was dependent on the motor protein dynein, suggesting a possible link between the modulation of microtubule dynamics at the cortex and dynein-dependent force production. EFA-6 orthologues activate ARF6-type GTPases to regulate vesicle trafficking. However, we show that only the C. elegans EFA-6 amino-terminus is both necessary and sufficient to limit microtubule growth along the cortex, and that this function is independent of ARF-6.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.