ArticleNucleic acids research2011
NLP is a novel transcription regulator involved in VSG expression site control in Trypanosoma brucei.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 37 citations in OpenAlex.
- Unwrap RAP1's Mystery at Kinetoplastid Telomeres.Biomolecules · 2024Review
- Stage-specific transcription activator ESB1 regulates monoallelic antigen expression in Trypanosoma brucei.Nature microbiology · 2022Article
- TbSAP is a novel chromatin protein repressing metacyclic variant surface glycoprotein expression sites in bloodstream form Trypanosoma brucei.Nucleic acids research · 2021Article
- Article
- May the Odds Be Ever in Your Favor: Non-deterministic Mechanisms Diversifying Cell Surface Molecule Expression.Frontiers in cell and developmental biology · 2021Review
- Dynamic colocalization of 2 simultaneously activeProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Review
- Transcriptional Regulation of Telomeric Expression Sites and Antigenic Variation in Trypanosomes.Current genomics · 2018Review
- The role of genomic location and flanking 3'UTR in the generation of functional levels of variant surface glycoprotein in Trypanosoma brucei.Molecular microbiology · 2017Article
- The VEXing problem of monoallelic expression in the African trypanosome.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Epigenetic Regulation of Virulence Gene Expression in Parasitic Protozoa.Cell host & microbe · 2016Review
- Identification of the ISWI Chromatin Remodeling Complex of the Early Branching Eukaryote Trypanosoma brucei.The Journal of biological chemistry · 2015Article
- DNA double-strand breaks and telomeres play important roles in trypanosoma brucei antigenic variation.Eukaryotic cell · 2015Review
- Review
- FACT plays a major role in histone dynamics affecting VSG expression site control in Trypanosoma brucei.Molecular microbiology · 2014Article
- Trypanosoma brucei histone H1 inhibits RNA polymerase I transcription and is important for parasite fitness in vivo.Molecular microbiology · 2014Article
- Transcription is initiated on silent variant surface glycoprotein expression sites despite monoallelic expression in Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Promoter occupancy of the basal class I transcription factor A differs strongly between active and silent VSG expression sites in Trypanosoma brucei.Nucleic acids research · 2014Article
- Antigenic variation in African trypanosomes: the importance of chromosomal and nuclear context in VSG expression control.Cellular microbiology · 2013Review
- Silencing subtelomeric VSGs by Trypanosoma brucei RAP1 at the insect stage involves chromatin structure changes.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Trypanosoma brucei mono-allelically expresses one of approximately 1500 variant surface glycoprotein (VSG) genes while multiplying in the mammalian bloodstream. The active VSG is transcribed by RNA polymerase I in one of approximately 15 telomeric VSG expression sites (ESs). T. brucei is unusual in controlling gene expression predominantly post-transcriptionally, and how ESs are mono-allelically controlled remains a mystery. Here we identify a novel transcription regulator, which resembles a nucleoplasmin-like protein (NLP) with an AT-hook motif. NLP is key for ES control in bloodstream form T. brucei, as NLP knockdown results in 45- to 65-fold derepression of the silent VSG221 ES. NLP is also involved in repression of transcription in the inactive VSG Basic Copy arrays, minichromosomes and procyclin loci. NLP is shown to be enriched on the 177- and 50-bp simple sequence repeats, the non-transcribed regions around rDNA and procyclin, and both active and silent ESs. Blocking NLP synthesis leads to downregulation of the active ES, indicating that NLP plays a role in regulating appropriate levels of transcription of ESs in both their active and silent state. Discovery of the unusual transcription regulator NLP provides new insight into the factors that are critical for ES control.
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Registered trials
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