ArticleBiology of reproduction2011
Transcript profiling of individual twin blastomeres derived by splitting two-cell stage murine embryos.
Article in Biology of reproduction, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Serotonin has early, cilia-independent roles in Xenopus left-right patterning.Disease models & mechanisms · 2013Pooled it
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- Induction of Stress Granules and Developmental Instability of Offspring Phenotype Due to Hypothermia During First Mouse Embryo Cleavage.International journal of molecular sciences · 2025Article
- Cellular mechanisms of monozygotic twinning: clues from assisted reproduction.Human reproduction update · 2024Review
- Characterization of Enlarged Tongues in Cloned Piglets.Current issues in molecular biology · 2023Article
- cd26 Knockdown Negatively Affects Porcine Parthenogenetic Preimplantation Embryo Development.Animals : an open access journal from MDPI · 2022Article
- Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos.Scientific reports · 2021Article
- Totipotency segregates between the sister blastomeres of two-cell stage mouse embryos.Scientific reports · 2017Article
- From cytoskeletal dynamics to organ asymmetry: a nonlinear, regulative pathway underlies left-right patterning.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- The Role of Maternal-Effect Genes in Mammalian Development: Are Mammalian Embryos Really an Exception?Stem cell reviews and reports · 2016Review
- Article
- Totipotency: what it is and what it is not.Stem cells and development · 2014Article
- It's never too early to get it Right: A conserved role for the cytoskeleton in left-right asymmetry.Communicative & integrative biology · 2013Article
- A unified model for left-right asymmetry? Comparison and synthesis of molecular models of embryonic laterality.Developmental biology · 2013Review
- Early, nonciliary role for microtubule proteins in left-right patterning is conserved across kingdoms.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
In invertebrates and amphibians, informational macromolecules in egg cytoplasm are organized to provide direction to the formation of embryonic lineages, but it is unclear whether vestiges of such prepatterning exist in mammals. Here we examined whether twin blastomeres from 2-cell stage mouse embryos differ in mRNA content. mRNA from 26 blastomeres derived from 13 embryos approximately mid-way through their second cell cycle was subjected to amplification. Twenty amplified samples were hybridized to arrays. Of those samples that hybridized successfully, 12 samples in six pairs were used in the final analysis. Probes displaying normalized values >0.25 (n = 4573) were examined for consistent bias in expression within blastomere pairs. Although transcript content varied between both individual embryos and twin blastomeres, no consistent asymmetries were observed for the majority of genes, with only 178 genes displaying a >1.4-fold difference in expression across all six pairs. Although class discovery clustering showed that blastomere pairs separated into two distinct groups in terms of their differentially expressed genes, when the data were tested for significance of asymmetrical expression, only 39 genes with >1.4-fold change ratios in six of six blastomere pairs passed the two-sample t-test (P < 0.05). Transcripts encoding proteins implicated in RNA processing and cytoskeletal organization were among the most abundant, differentially distributed mRNA, suggesting that a stochastically based lack of synchrony in cell cycle progression between the two cells might explain at least some and possibly all of the asymmetries in transcript composition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.