ArticleJournal of bacteriology2011
Genetic and mass spectrometry analyses of the unusual type IV-like pili of the archaeon Methanococcus maripaludis.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 63 citations in OpenAlex.
- N-glycosylation in Archaea - Expanding the process, components and roles of a universal post-translational modification.BBA advances · 2024Article
- Characterization of protein glycosylation in an Asgard archaeon.BBA advances · 2024Article
- Model Organisms To Study Methanogenesis, a Uniquely Archaeal Metabolism.Journal of bacteriology · 2023Review
- The Oligosaccharyltransferase AglB Supports Surface-Associated Growth and Iron Oxidation in Methanococcus maripaludis.Applied and environmental microbiology · 2021Article
- Identification of a novelThe Journal of biological chemistry · 2020Article
- Type IV-Like Pili Facilitate Transformation in Naturally Competent Archaea.Journal of bacteriology · 2020Article
- High-resolution archaellum structure reveals a conserved metal-binding site.EMBO reports · 2019Article
- Archaeal cell surface biogenesis.FEMS microbiology reviews · 2018Article
- Metabolic processes of Methanococcus maripaludis and potential applications.Microbial cell factories · 2016Review
- Identification of Haloferax volcanii Pilin N-Glycans with Diverse Roles in Pilus Biosynthesis, Adhesion, and Microcolony Formation.The Journal of biological chemistry · 2016Article
- Functional analysis of N-linking oligosaccharyl transferase enzymes encoded by deep-sea vent proteobacteria.Glycobiology · 2016Article
- Complementation of an aglB Mutant of Methanococcus maripaludis with Heterologous Oligosaccharyltransferases.PloS one · 2016Article
- Diversity and Evolution of Type IV pili Systems in Archaea.Frontiers in microbiology · 2016Article
- Evidence that biosynthesis of the second and third sugars of the archaellin Tetrasaccharide in the archaeon Methanococcus maripaludis occurs by the same pathway used by Pseudomonas aeruginosa to make a di-N-acetylated sugar.Journal of bacteriology · 2015Article
- Pilin Processing Follows a Different Temporal Route than That of Archaellins in Methanococcus maripaludis.Life (Basel, Switzerland) · 2015Article
- Editorial.FEMS microbiology reviews · 2015Article
- Archaeal type IV pili and their involvement in biofilm formation.Frontiers in microbiology · 2015Article
- Effects of N-glycosylation site removal in archaellins on the assembly and function of archaella in Methanococcus maripaludis.PloS one · 2015Article
- Exceptionally widespread nanomachines composed of type IV pilins: the prokaryotic Swiss Army knives.FEMS microbiology reviews · 2015Review
- Dark matter in archaeal genomes: a rich source of novel mobile elements, defense systems and secretory complexes.Extremophiles : life under extreme conditions · 2014Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structure of pili from the archaeon Methanococcus maripaludis is unlike that of any bacterial pili. However, genetic analysis of the genes involved in the formation of these pili has been lacking until this study. Pili were isolated from a nonflagellated (ΔflaK) mutant and shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to consist primarily of subunits with an apparent molecular mass of 17 kDa. In-frame deletions were created in three genes, MMP0233, MMP0236, and MMP0237, which encode proteins with bacterial type IV pilin-like signal peptides previously identified by in silico methodology as likely candidates for pilus structural proteins. Deletion of MMP0236 or MMP0237 resulted in mutant cells completely devoid of pili on the cell surface, while deletion of the third pilin-like gene, MMP0233, resulted in cells greatly reduced in the number of pili on the surface. Complementation with the deleted gene in each case returned the cells to a piliated state. Surprisingly, mass spectrometry analysis of purified pili identified the major structural pilin as another type IV pilin-like protein, MMP1685, whose gene is located outside the first pilus locus. This protein was found to be glycosylated with an N-linked branched pentasaccharide glycan. Deletion and complementation analysis confirmed that MMP1685 is required for piliation.
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