ArticleApplied and environmental microbiology2011
Real-time PCR assay to differentiate Listeriolysin S-positive and -negative strains of Listeria monocytogenes.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 63 citations in OpenAlex.
- Prevalence and Antimicrobial Resistance ofFoods (Basel, Switzerland) · 2024Article
- Genomic and pathogenicity islands ofFrontiers in molecular biosciences · 2023Review
- Prevalence and transmission characteristics ofEmerging microbes & infections · 2021Article
- Listeriolysin S: A bacteriocin fromProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Multiplex and on-site PCR detection of swine diseases based on the microfluidic chip system.BMC veterinary research · 2021Article
- Whole Genome-Based Characterization ofFrontiers in microbiology · 2021Article
- Bacteriocins ofToxins · 2020Article
- Heterogeneity, Characteristics, and Public Health Implications ofFrontiers in microbiology · 2020Article
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- Genetic characteristics and virulence of Listeria monocytogenes isolated from fresh vegetables in China.BMC microbiology · 2019Article
- Association of Listeria monocytogenes LIPI-1 and LIPI-3 marker llsX with invasiveness.Current microbiology · 2019Article
- Isolation, Potential Virulence, and Population Diversity ofFrontiers in microbiology · 2019Article
- Genomic Diversity ofGenes · 2018Article
- Prevalence and molecular characteristics of Listeria monocytogenes in cooked products and its comparison with isolates from listeriosis cases.Frontiers of medicine · 2018Article
- Occurrence, Antibiotic Resistance, and Population Diversity ofFrontiers in microbiology · 2018Article
- Prevalence, Potential Virulence, and Genetic Diversity ofFrontiers in microbiology · 2018Article
- Listeriolysin S: A bacteriocin from epidemic Listeria monocytogenes strains that targets the gut microbiota.Gut microbes · 2017Article
- YcaO-Dependent Posttranslational Amide Activation: Biosynthesis, Structure, and Function.Chemical reviews · 2017Review
- Bacteriocin from epidemic Listeria strains alters the host intestinal microbiota to favor infection.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to the severity of the food-borne infection listeriosis, strict legislation governs the detectable and permissible limits at which Listeria monocytogenes is permitted in foods. These requirements, coupled with the ubiquitous nature of L. monocytogenes strains and the potential for epidemic outbreaks, mean that the pathogen can devastate affected sectors of the food industry. Although almost all L. monocytogenes strains have the potential to cause listeriosis, those implicated in the vast majority of epidemics belong to a subset of strains belonging to evolutionary lineage I. It has been established that a significant proportion of these strains, including those implicated in the majority of outbreaks, produce an additional hemolysin, designated listeriolysin S (LLS), which may be responsible for the enhanced virulence of these strains. In order to ultimately establish this definitively, it is important to first be able to rapidly discriminate between LLS-positive and -negative strains. Here, after essential genes within the LLS-encoding cluster, Listeria pathogenicity island 3, were identified by deletion mutagenesis, a real-time PCR assay which targets one such gene, llsX, was developed as a means of identifying LLS-positive L. monocytogenes. The specificity of the assay was validated against a panel of 40 L. monocytogenes strains (20 of which were LLS positive) and 25 strains representative of other Listeria species. Furthermore, 1 CFU of an LLS-positive strain per 25 g/ml of spiked foods was detected in less than 30 h when the assay was coupled with culture enrichment. The detection limit of this assay was 10 genome equivalents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.