ArticleApplied and environmental microbiology2011
Transformation system for Hypocrea jecorina (Trichoderma reesei) that favors homologous integration and employs reusable bidirectionally selectable markers.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 143 citations in OpenAlex.
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- Discovery of the antifungal compound ilicicolin K through genetic activation of the ilicicolin biosynthetic pathway in Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2025Article
- Considerations for Domestication of Novel Strains of Filamentous Fungi.ACS synthetic biology · 2025Review
- Simply cut out - Combining CRISPR/Cas9 RNPs and transiently selected telomere vectors for marker free-gene deletion inFrontiers in genome editing · 2025Article
- Life on the rocks: unexpected enzyme activity of the extremophilic black fungusFrontiers in bioengineering and biotechnology · 2025Article
- An efficient CRISPR/Cas9 genome editing system based on a multiple sgRNA processing platform in Trichoderma reesei for strain improvement and enzyme production.Biotechnology for biofuels and bioproducts · 2024Article
- Establishment, optimization, and application of genetic technology inFrontiers in microbiology · 2023Review
- Gramiketides, Novel Polyketide Derivatives ofJournal of fungi (Basel, Switzerland) · 2022Article
- A three-gene cluster in Trichoderma reesei reveals a potential role of dmm2 in DNA repair and cellulase production.Biotechnology for biofuels and bioproducts · 2022Article
- CPExtract, a Software Tool for the Automated Tracer-Based Pathway Specific Screening of Secondary Metabolites in LC-HRMS Data.Analytical chemistry · 2022Article
- Transcriptional Activation of Biosynthetic Gene Clusters in Filamentous Fungi.Frontiers in bioengineering and biotechnology · 2022Review
- An overview on current molecular tools for heterologous gene expression in Trichoderma.Fungal biology and biotechnology · 2021Review
- Article
- Generation of Trichoderma harzianum with pyr4 auxotrophic marker by using the CRISPR/Cas9 system.Scientific reports · 2021Article
- Genetic Transformation of Trichoderma spp.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Nanoscale Infrared Spectroscopy and Chemometrics Enable Detection of Intracellular Protein Distribution.Analytical chemistry · 2020Article
- Novel approach in whole genome mining and transcriptome analysis reveal conserved RiPPs in Trichoderma spp.BMC genomics · 2020Article
- Regulation of gene expression by the action of a fungal lncRNA on a transactivator.RNA biology · 2020Article
- The Kinase USK1 Regulates Cellulase Gene Expression and Secondary Metabolite Biosynthesis inFrontiers in microbiology · 2020Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Hypocrea jecorina is an industrially important filamentous fungus due to its effective production of hydrolytic enzymes. It has received increasing interest because of its ability to convert lignocellulosic biomass to monomeric sugars, which can be converted into biofuels or platform chemicals. Genetic engineering of strains is a highly important means of meeting the requirements of tailor-made applications. Therefore, we report the development of a transformation system that allows highly efficient gene targeting by using a tmus53 (human LIG4 homolog) deletion strain. Moreover, it permits the unlimited reuse of the same marker by employing a Cre/loxP-based excision system. Both marker insertion and marker excision can be positively selected for by combining resistance to hygromycin B and loss of sensitivity to fluoroacetamide. Finally, the marker pyr4, also positively selectable for insertion and loss, can be used to remove the cre gene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.