ArticleApplied and environmental microbiology2011
Transformation of, and heterologous protein expression in, Lactobacillus agilis and Lactobacillus vaginalis isolates from the chicken gastrointestinal tract.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- L. AGILIS ALLEVIATES SALPINGITIS IN HENS Lactobacillus agilis alleviates salpingitis through the TLR4/MyD88/NF-κB pathway and gut microbiota interactions in aged laying hens.Poultry science · 2026Article
- Development of the Efficient Electroporation Protocol forInternational journal of molecular sciences · 2025Article
- Advances in Genetic Transformation of Lactic Acid Bacteria: Overcoming Barriers and Enhancing Plasmid Tools.International journal of molecular sciences · 2025Review
- Harnessing flagellin ofApplied and environmental microbiology · 2025Article
- Modified vaginal lactobacilli expressing fluorescent and luminescent proteins for more effective monitoring of their release from nanofibers, safety and cell adhesion.Microbial cell factories · 2024Article
- Co-expression of endoglucanase and cellobiohydrolase from yak rumen in lactic acid bacteria and its preliminary application in whole-plant corn silage fermentation.Frontiers in microbiology · 2024Article
- Expression of β-Glucosidases from the Yak Rumen in Lactic Acid Bacteria: A Genetic Engineering Approach.Microorganisms · 2023Article
- Immunogenic Modification of Ligilactobacillus agilis by Specific Amino Acid Substitution of Flagellin.Applied and environmental microbiology · 2022Article
- Engineering of Vaginal Lactobacilli to Express Fluorescent Proteins Enables the Analysis of Their Mixture in Nanofibers.International journal of molecular sciences · 2021Article
- Isolation and Identification of a RumenMicroorganisms · 2021Article
- Stable Recombinant-Gene Expression from aApplied and environmental microbiology · 2021Article
- Conjugative DNA Transfer FromFrontiers in microbiology · 2021Article
- Cloning and functional expression of a food-grade circular bacteriocin, plantacyclin B21AG, in probiotic Lactobacillus plantarum WCFS1.PloS one · 2020Article
- The impact of motility on the localization of Lactobacillus agilis in the murine gastrointestinal tract.BMC microbiology · 2018Article
- Characterization of flagellins isolated from a highly motile strain of Lactobacillus agilis.BMC microbiology · 2016Article
- Coexpression and secretion of endoglucanase and phytase genes in Lactobacillus reuteri.International journal of molecular sciences · 2014Article
- A murine oral model for Mycobacterium avium subsp. paratuberculosis infection and immunomodulation with Lactobacillus casei ATCC 334.Frontiers in cellular and infection microbiology · 2014Article
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3 authors.
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Abstract
Lactobacilli are naturally found in the gastrointestinal tract of chickens, and there is interest in utilizing autochthonous strains for the delivery of therapeutic proteins. Previously we identified three chicken-derived Lactobacillus strains, Lactobacillus agilis La3, Lactobacillus vaginalis Lv5, and Lactobacillus crispatus Lc9, which persist in the gastrointestinal tract of chickens fed either a commercial or high-protein diet. In the current study, we investigated the ability to electrotransform these strains, determined plasmid vector stability, and compared reporter gene expression directed by several different promoters. The La3 and Lv5 strains were reproducibly transformed with efficiencies of 10(8) and 10(6) transformants per microgram of plasmid DNA, respectively. The third strain tested, L. crispatus Lc9, was recalcitrant to all transformation protocols examined. The plasmid vectors pTRK563 and pTRKH2 were maintained over 100 generations in La3 and Lv5, respectively. The ability of La3 and Lv5 to express the heterologous reporter gene gfp was analyzed using heterologous and homologous promoters. Transformants of both La3 and Lv5 containing the La3 ldhL promoter were the most fluorescent. To our knowledge, this is the first report of successful transformation and heterologous protein expression in L. agilis and L. vaginalis. The ability of these strains to express heterologous proteins in vitro indicates their potential utility as in vivo delivery vectors for therapeutic peptides to the chicken gastrointestinal tract.
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