ArticleNucleic acids research2011
Mismatch-repair protein MSH6 is associated with Ku70 and regulates DNA double-strand break repair.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 65 citations in OpenAlex.
- Dysfunctional DNA Mismatch Repair Drives the Evolution of Gene Amplification in MTX-Resistant Human Colorectal Cancer Cells.International journal of molecular sciences · 2026Article
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- TDP43 interacts with MLH1 and MSH6 proteins in a DNA damage-inducible manner.Molecular brain · 2024Article
- TDP43 Interacts with MLH1 and MSH6 Proteins in A DNA Damage-Inducible Manner.Research square · 2024Article
- Article
- Novel insights into the ecDNA formation mechanism involving MSH3 in methotrexate‑resistant human colorectal cancer cells.International journal of oncology · 2023Article
- Widespread BRCA1/2-independent homologous recombination defects are caused by alterations in RNA-binding proteins.Cell reports. Medicine · 2023Article
- Nuclear DJ-1 Regulates DNA Damage Repair via the Regulation of PARP1 Activity.International journal of molecular sciences · 2023Article
- Article
- Article
- CNOT6: A Novel Regulator of DNA Mismatch Repair.Cells · 2022Article
- An integrative pan-cancer analysis reveals the oncogenic role of mutS homolog 6 (MSH6) in human tumors.Aging · 2021Article
- Alteration of protein expression and spliceosome pathway activity during Barrett's carcinogenesis.Journal of gastroenterology · 2021Article
- An SR protein is essential for activating DNA repair in malaria parasites.Journal of cell science · 2021Article
- Article
- Two-way crosstalk between BER and c-NHEJ repair pathway is mediated by Pol-β and Ku70.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Temozolomide Induced Hypermutation in Glioma: Evolutionary Mechanisms and Therapeutic Opportunities.Frontiers in oncology · 2019Review
- Article
- Divergent roles for Clusterin in Lung Injury and Repair.Scientific reports · 2017Article
- CSE1L interaction with MSH6 promotes osteosarcoma progression and predicts poor patient survival.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MSH6, a key component of the MSH2-MSH6 complex, plays a fundamental role in the repair of mismatched DNA bases. Herein, we report that MSH6 is a novel Ku70-interacting protein identified by yeast two-hybrid screening. Ku70 and Ku86 are two key regulatory subunits of the DNA-dependent protein kinase, which plays an essential role in repair of DNA double-strand breaks (DSBs) through the non-homologous end-joining (NEHJ) pathway. We found that association of Ku70 with MSH6 is enhanced in response to treatment with the radiomimetic drug neocarzinostatin (NCS) or ionizing radiation (IR), a potent inducer of DSBs. Furthermore, MSH6 exhibited diffuse nuclear staining in the majority of untreated cells and forms discrete nuclear foci after NCS or IR treatment. MSH6 colocalizes with γ-H2AX at sites of DNA damage after NCS or IR treatment. Cells depleted of MSH6 accumulate high levels of persistent DSBs, as detected by formation of γ-H2AX foci and by the comet assay. Moreover, MSH6-deficient cells were also shown to exhibit impaired NHEJ, which could be rescued by MSH6 overexpression. MSH6-deficient cells were hypersensitive to NCS- or IR-induced cell death, as revealed by a clonogenic cell-survival assay. These results suggest a potential role for MSH6 in DSB repair through upregulation of NHEJ by association with Ku70.
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