ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2011
Singleplex real-time RT-PCR for detection of influenza A virus and simultaneous differentiation of A/H1N1v and evaluation of the RealStar influenza kit.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- Adaptation and validation of an influenza a subtyping panel for detection of H1pdm09, H3 and H5 on a high-throughput RT-qPCR system.Scientific reports · 2026Article
- The impact of the COVID-19 pandemic on other respiratory viruses in the Haut-Ogooué Province of Gabon.IJID regions · 2025Article
- A Rapid On-Site Assay for the Detection of Influenza A by Capillary Convective PCR.Molecular diagnosis & therapy · 2018Article
- A diagnostic one-step real-time reverse transcription polymerase chain reaction method for accurate detection of influenza virus type A.Archives of medical science : AMS · 2016Article
- Viral etiology and seasonality of influenza-like illness in Gabon, March 2010 to June 2011.BMC infectious diseases · 2014Article
- Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009/10.Archives of virology · 2014Article
- Acute fibrinous and organizing pneumonia associated with influenza A/H1N1 pneumonia after lung transplantation.BMC pulmonary medicine · 2013Article
- [Influenza today and in the future].Der Pneumologe · 2013Article
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA novel influenza A virus, subtype A/H1N1v emerged in April 2009 and caused the first influenza pandemic of the 21st century. Reliable detection and differentiation from seasonal influenza viruses is mandatory for appropriate case management as well as public health.
objectivesTo develop and technically validate a novel one-step real-time RT-PCR assay which can be used for influenza A virus screening and subtyping of A/H1N1v in a singleplex fashion. To assess the clinical performance of a novel commercial influenza RT-PCR kit based on the in-house version. STUDY
designA real-time RT-PCR assay targeting the matrix gene of influenza A viruses was developed and validated using in vitro transcribed RNA derived from influenza A/H1N1v, A/H1N1 and A/H3N2 virus as well as plaque-quantified influenza A/H1N1v, A/H1N1 and A/H3N2 virus samples. After validation of the in-house version the commercial RealStar kit was used to assess the clinical performance and specificity on a panel of influenza viruses including A/H1N1v, A/H1N1, swine A/H1N1, A/H3N2, avian A/H5N1 as well as patient specimens.
resultsThe lower limit of detection of the in-house version was 2149, 1376 and 2994 RNA copies/ml for A/H1N1v, A/H1N1 and A/H3N2, respectively. The RealStar kit displayed 100% sensitivity and specificity and could reliably discriminate influenza A viruses from A/H1N1v. No cross reaction with swine A/H1N1 and A/H1N2 was observed with the RealStar A/H1N1v specific probe.
conclusionBoth assays demonstrated high sensitivity and specificity and might assist in the diagnosis of suspected influenza cases.
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