Trial reportThe Journal of urology2011
A biosensor platform for rapid antimicrobial susceptibility testing directly from clinical samples.
Trial report in The Journal of urology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed.
- Next-generation rapid phenotypic antimicrobial susceptibility testing.Nature communications · 2024Review
- Microfluidic technologies for advanced antimicrobial susceptibility testing.Biomicrofluidics · 2024Review
- Assessing the impact of discordant antibiotic treatment on adverse outcomes in community-onset UTI: a retrospective cohort study.The Journal of antimicrobial chemotherapy · 2024Article
- Current and Future Technologies for the Detection of Antibiotic-Resistant Bacteria.Diagnostics (Basel, Switzerland) · 2023Review
- Real world clinical feasibility of direct-from-specimen antimicrobial susceptibility testing of clinical specimens with unknown microbial load or susceptibility.Scientific reports · 2022Article
- Combating Antimicrobial Resistance via Single-Cell Diagnostic Technologies Powered by Droplet Microfluidics.Accounts of chemical research · 2022Article
- Microfluidic Systems for Antimicrobial Susceptibility Testing.Advances in biochemical engineering/biotechnology · 2022Article
- Article
- Nanomotion Detection-Based Rapid Antibiotic Susceptibility Testing.Antibiotics (Basel, Switzerland) · 2021Review
- A rapid, antibiotic susceptibility test for multidrug-resistant, Gram-negative bacterial uropathogens using the biochemical assay, DETECT.Journal of microbiological methods · 2021Article
- Droplet-Based Single-Cell Measurements of 16S rRNA Enable Integrated Bacteria Identification and Pheno-Molecular Antimicrobial Susceptibility Testing from Clinical Samples in 30 min.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species.Frontiers in bioengineering and biotechnology · 2021Article
- Nanofluidic Immobilization and Growth Detection ofBiosensors · 2020Article
- Rapid Detection and Antibiotic Susceptibility of UropathogenicMicroorganisms · 2020Article
- Surfactant-enhanced DNA accessibility to nuclease accelerates phenotypic β-lactam antibiotic susceptibility testing of Neisseria gonorrhoeae.PLoS biology · 2020Article
- Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae.PLoS biology · 2020Article
- 3D microfluidic gradient generator for combination antimicrobial susceptibility testing.Microsystems & nanoengineering · 2020Article
- Modern Tools for Rapid Diagnostics of Antimicrobial Resistance.Frontiers in cellular and infection microbiology · 2020Review
- Adaptable microfluidic system for single-cell pathogen classification and antimicrobial susceptibility testing.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Optimizing peptide nucleic acid probes for hybridization-based detection and identification of bacterial pathogens.The Analyst · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
purposeA significant barrier to efficient antibiotic management of infection is that the standard diagnostic methodologies do not provide results at the point of care. The delays between sample collection and bacterial culture and antibiotic susceptibility reporting have led to empirical use of antibiotics, contributing to the emergence of drug resistant pathogens. As a key step toward the development of a point of care device for determining the antibiotic susceptibility of urinary tract pathogens, we report on a biosensor based antimicrobial susceptibility test. MATERIALS AND
methodsFor assay development bacteria were cultured with or without antibiotics, and growth was quantitated by determining viable counts and electrochemical biosensor measurement of bacterial 16S rRNA. To determine antibiotic susceptibility directly from patient samples, urine was cultured on antibiotic plates for 2.5 hours and growth was determined by electrochemical measurement of bacterial 16S rRNA. For assay validation 252 urine samples were collected from patients at the Spinal Cord Injury Service at Veterans Affairs Palo Alto Health Care System. The biosensor based antimicrobial susceptibility test was completed for samples containing gram-negative organisms. Pathogen identification and antibiotic susceptibility results were compared between our assay and standard microbiological analysis.
resultsA direct biosensor quantitation of bacterial 16S rRNA can be used to monitor bacterial growth for a biosensor based antimicrobial susceptibility test. Clinical validation of a biosensor based antimicrobial susceptibility test with patient urine samples demonstrated that this test was 94% accurate in 368 pathogen-antibiotic tests compared to standard microbiological analysis.
conclusionsThis biosensor based antimicrobial susceptibility test, in concert with our previously described pathogen identification assay, can provide culture and susceptibility information directly from a urine sample within 3.5 hours.
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