ArticleApplied spectroscopy2010
Macromolecular concentrations in bovine nasal cartilage by Fourier transform infrared imaging and principal component regression.
Article in Applied spectroscopy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Express Analysis of Cartilage Tissue Using Multivariate Analysis of IR Spectra.Sovremennye tekhnologii v meditsine · 2022Article
- Established method of chondroitin sulphate extraction from buffalo (Journal of food science and technology · 2018Article
- Proteoglycan concentrations in healthy and diseased articular cartilage by Fourier transform infrared imaging and principal component regression.Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2014Article
- Structure-function relations and rigidity percolation in the shear properties of articular cartilage.Biophysical journal · 2014Article
- Near infrared spectroscopic evaluation of water in hyaline cartilage.Annals of biomedical engineering · 2013Article
- Fourier-transform infrared spectroscopic imaging of articular cartilage and biomaterials: A review.Trends in applied spectroscopy · 2013Article
- MRI of articular cartilage at microscopic resolution.Bone & joint research · 2013Article
- Matrix disruptions, growth, and degradation of cartilage with impaired sulfation.The Journal of biological chemistry · 2012Article
- Concentration profiles of collagen and proteoglycan in articular cartilage by Fourier transform infrared imaging and principal component regression.Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2012Article
- Article
- Depth-dependent Anisotropy of Proteoglycan in Articular Cartilage by Fourier Transform Infrared Imaging.Vibrational Spectroscopy · 2011Article
- Chemical visualization of individual chondrocytes in articular cartilage by attenuated-total-reflection Fourier Transform Infrared Microimaging.Biomedical optics express · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Fourier transform infrared imaging (FT-IRI) and principal component regression (PCR) were used to quantitatively determine collagen and proteoglycan concentrations in bovine nasal cartilage (BNC). An infrared spectral library was first established by obtaining eleven infrared spectra from a series of collagen and chondroitin 6-sulfate mixed in different ratios. FT-IR images were obtained from 6-μm-thick sections of BNC specimens at 6.25-μm pixel size. The spectra from the FT-IR images were imported into a PCR program to obtain the relative concentrations of collagen and proteoglycan in BNC, based on the spectral library of pure chemicals. These PCR-determined concentrations agreed with the molecular concentrations determined biochemically using an enzyme digestion assay. Use of the imaging approach revealed that proteoglycan loss in the specimens occurs first at the surface of the tissue block when compared with the middle portion of the tissue block. The quantitative correlation of collagen and proteoglycan revealed that their infrared absorption peak areas at 1338 and 1072-855 cm(-1) can only be used as qualitative indicators of the molecular contents. The use of PCR with FT-IRI offers an accurate tool to spatially determine the distributions of macromolecular concentration in cartilage.
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Registered trials
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